 |
English
|
正體中文
|
简体中文
|
全文筆數/總筆數 : 46867/50733 (92%)
造訪人次 : 11887732
線上人數 : 760
|
|
|
資料載入中.....
|
請使用永久網址來引用或連結此文件:
https://irlib.pccu.edu.tw/handle/987654321/45478
|
| 題名: | Cloning and overexpression of the ascorbate peroxidase gene from the yam (Dioscorea alata) enhances chilling and flood tolerance in transgenic Arabidopsis |
| 作者: | Chen, ZH (Chen, Zhihua)
Lu, HH (Lu, Hsueh-Han)
Hua, SM (Hua, Shumei)
Lin, KH (Lin, Kuan-Hung)
Chen, ND (Chen, Ningdan)
Zhang, YW (Zhang, Yangwen)
You, ZY (You, Ziyi)
Kuo, YW (Kuo, Yun-Wei)
Chen, SP (Chen, Shi-Peng) |
| 貢獻者: | 園生系 |
| 關鍵詞: | Ascorbate peroxidase
Chilling tolerance
Dioscorea alata
Flood tolerance
Transgenic Arabidopsis |
| 日期: | 2019-11 |
| 上傳時間: | 2019-12-13 11:00:06 (UTC+8) |
| 摘要: | Minghuai 1 (MH1) is a yam (Dioscorea alata) cultivar with high tolerance to flooding but sensitivity to chilling. MH1 responded differently to chilling and flooding according to various physiological parameters and antioxidant enzymes. Flooding led to an increase in ascorbate peroxidase (APX) activity in both roots and leaves, while chilling did not affect APX activity. The full length DaAPX ORF sequence from MH1 (750 bp) was then cloned. Phylogenetic analysis showed that plant cytosolic APXs into four major clusters and DaAPX was closely related to Oncidium. The DaAPX gene driven by a 35S promoter was transferred into Arabidopsis. The gene expression and enzyme activity of APX in the DaAPX transgenic lines 1-3 were significantly higher than in wild type (WT) plants. Compared to WT plants, seedling growth characteristics were significantly better in all transgenic lines under chilling, flooding, and oxidative stresses, indicating that the overexpression of DaAPX in Arabidopsis enhanced tolerance to several abiotic stresses. MH1 plants supplied with H2O2 presented an increase in the activity of APX leading to enhanced tolerance to chilling. Functional characterization of the APX gene should improve our understanding of the chilling- and flood-response mechanism in the yam. |
| 關聯: | JOURNAL OF PLANT RESEARCH 卷冊: 132 期: 6 頁數: 857-866 |
| 顯示於類別: | [園藝暨生物技術學系] 期刊論文
|
文件中的檔案:
| 檔案 |
描述 |
大小 | 格式 | 瀏覽次數 |
|---|
| index.html | | 0Kb | HTML | 313 | 檢視/開啟 |
|
在CCUR中所有的資料項目都受到原著作權保護.
|
