文化大學機構典藏 CCUR:Item 987654321/45478
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    题名: Cloning and overexpression of the ascorbate peroxidase gene from the yam (Dioscorea alata) enhances chilling and flood tolerance in transgenic Arabidopsis
    作者: Chen, ZH (Chen, Zhihua)
    Lu, HH (Lu, Hsueh-Han)
    Hua, SM (Hua, Shumei)
    Lin, KH (Lin, Kuan-Hung)
    Chen, ND (Chen, Ningdan)
    Zhang, YW (Zhang, Yangwen)
    You, ZY (You, Ziyi)
    Kuo, YW (Kuo, Yun-Wei)
    Chen, SP (Chen, Shi-Peng)
    贡献者: 園生系
    关键词: Ascorbate peroxidase
    Chilling tolerance
    Dioscorea alata
    Flood tolerance
    Transgenic Arabidopsis
    日期: 2019-11
    上传时间: 2019-12-13 11:00:06 (UTC+8)
    摘要: Minghuai 1 (MH1) is a yam (Dioscorea alata) cultivar with high tolerance to flooding but sensitivity to chilling. MH1 responded differently to chilling and flooding according to various physiological parameters and antioxidant enzymes. Flooding led to an increase in ascorbate peroxidase (APX) activity in both roots and leaves, while chilling did not affect APX activity. The full length DaAPX ORF sequence from MH1 (750 bp) was then cloned. Phylogenetic analysis showed that plant cytosolic APXs into four major clusters and DaAPX was closely related to Oncidium. The DaAPX gene driven by a 35S promoter was transferred into Arabidopsis. The gene expression and enzyme activity of APX in the DaAPX transgenic lines 1-3 were significantly higher than in wild type (WT) plants. Compared to WT plants, seedling growth characteristics were significantly better in all transgenic lines under chilling, flooding, and oxidative stresses, indicating that the overexpression of DaAPX in Arabidopsis enhanced tolerance to several abiotic stresses. MH1 plants supplied with H2O2 presented an increase in the activity of APX leading to enhanced tolerance to chilling. Functional characterization of the APX gene should improve our understanding of the chilling- and flood-response mechanism in the yam.
    關聯: JOURNAL OF PLANT RESEARCH 卷冊: 132 期: 6 頁數: 857-866
    显示于类别:[園藝暨生物技術學系] 期刊論文

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