蟲癭是擴展表型現象中非常特別的例子，雖然癭靠寄主植物組織而構成，但是調控機制卻受到昆蟲的影響。本研究室利用NGS技術應用在蟲癭上進行新基因轉錄體定序，結果顯示有863個差異表現基因，其中包含細胞壁合成、植物生長激素、光合作用、二次代謝、四吡咯生合成、植物逆境等類群。此外，大葉楠蟲癭和其寄主葉片的小分子代謝物分析中，結果顯示有數個蟲癭組織和葉片差異的小分子化合物，值得進一步取樣進行更多不同的代謝產物分析。本計畫將利用大葉楠蟲癭轉錄體研究之結果，利用RT-qPCR、生化分析及代謝體分析進一步探討不同發育時期蟲癭之基因表現、生理差異、代謝體變化及天然物成分。
Insect gall is a very special case of the extended phenotypes. The development of galls depend on the host plant tissue, but the regulation mechanism is affected by insects. Our pervious study used NGS to analysis transcriptome de novo sequencing on galls. The results showed that there were 863 differently expressed genes, including cell wall synthesis, plant growth hormone, photosynthesis, secondary metabolism, Tetraploid pyrrole synthesis and plant stress. In addition, small molecular metabolites were found in the galls and leaves in Machilus japonica. The results showed several small molecular compounds in gall tissues and leaves, which were worthy of further sampling for more metabolites. This project will use the RT-qPCR, biochemistry and metabolome in research of the gene expression, physiological differences, metabolic changes and natural constituents of gall and leaves at different development stages.
