文化大學機構典藏 CCUR:Item 987654321/41928
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/41928


    题名: Efficient in vitro Plant Regeneration from Protocorm of Bletilla formosana (Hayata) Schltr.
    作者: Nguyen, MK (Minh-Khiem Nguyen)
    Lin, KH (Lin, Kuan-Hung)
    Chen, LR (Chen, Li-Ru)
    Hsiung, TC (Hsiung, Tung-Chuan)
    Ozaki, Y (Ozaki, Yukio)
    贡献者: 園藝暨生物技術學系
    关键词: MEDICINALLY IMPORTANT ORCHID
    CHEMICAL-CONSTITUENTS
    ANTIOXIDANT ACTIVITY
    CULTURE-MEDIA
    GROWTH
    SUCROSE
    PROPAGATION
    ROOTS
    日期: 2018
    上传时间: 2019-01-22 10:33:14 (UTC+8)
    摘要: An efficient mass propagation method for Bletilla. formosana (Hayata) Schltr. was successfully established through direct shoot organogenesis. Multiple shoots were induced from protocorm explants on half strength Murashige and Skoog (1/2-MS) basal medium containing 2 mg/L N-phenyl-N'-1,2,3-thiadiazol-5-yl urea (TDZ) and 0.5 mg/L l-naphthaleneacetic acid (NAA) with the highest shoot formation rate of 100% and a maximum average shoot number of 5.2. The application of either NAA or 2,4-clichlorophenoxy (2,4-D) significantly induced root induction from shoots, and 2 mg/L NAA provided the highest root formation rate of 100% and a maximum number of roots (4.4 per shoot). Supplementation with kinetin and benzylaminopurine (BAP) of 0.5 similar to 2.0 mg/L in 1/2 -MS basal medium significantly promoted plantlet growth. To optimize the in vitro development of plantlets, 1/2-MS basal medium with modified 1/4-strength nitrogen content, and 20 g/L sucrose was used. Well-developed plantlets were successfully acclimatized in a greenhouse with over a 90% survival rate. This effective protocol of in vitro plant regeneration through direct shoot organogenesis can be utilized for the mass propagation and germplasm conservation of B. formosana.
    显示于类别:[園藝暨生物技術學系] 期刊論文

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