Efficient in vitro Plant Regeneration from Protocorm of Bletilla formosana (Hayata) Schltr.

CCUR > College of Environmental Planning & Bioresources > College of Agriculture > Department of Horticulture > journal articles > Item 987654321/41928

Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/41928

Title:	Efficient in vitro Plant Regeneration from Protocorm of Bletilla formosana (Hayata) Schltr.
Authors:	Nguyen, MK (Minh-Khiem Nguyen) Lin, KH (Lin, Kuan-Hung) Chen, LR (Chen, Li-Ru) Hsiung, TC (Hsiung, Tung-Chuan) Ozaki, Y (Ozaki, Yukio)
Contributors:	園藝暨生物技術學系
Keywords:	MEDICINALLY IMPORTANT ORCHID CHEMICAL-CONSTITUENTS ANTIOXIDANT ACTIVITY CULTURE-MEDIA GROWTH SUCROSE PROPAGATION ROOTS
Date:	2018
Issue Date:	2019-01-22 10:33:14 (UTC+8)
Abstract:	An efficient mass propagation method for Bletilla. formosana (Hayata) Schltr. was successfully established through direct shoot organogenesis. Multiple shoots were induced from protocorm explants on half strength Murashige and Skoog (1/2-MS) basal medium containing 2 mg/L N-phenyl-N'-1,2,3-thiadiazol-5-yl urea (TDZ) and 0.5 mg/L l-naphthaleneacetic acid (NAA) with the highest shoot formation rate of 100% and a maximum average shoot number of 5.2. The application of either NAA or 2,4-clichlorophenoxy (2,4-D) significantly induced root induction from shoots, and 2 mg/L NAA provided the highest root formation rate of 100% and a maximum number of roots (4.4 per shoot). Supplementation with kinetin and benzylaminopurine (BAP) of 0.5 similar to 2.0 mg/L in 1/2 -MS basal medium significantly promoted plantlet growth. To optimize the in vitro development of plantlets, 1/2-MS basal medium with modified 1/4-strength nitrogen content, and 20 g/L sucrose was used. Well-developed plantlets were successfully acclimatized in a greenhouse with over a 90% survival rate. This effective protocol of in vitro plant regeneration through direct shoot organogenesis can be utilized for the mass propagation and germplasm conservation of B. formosana.
Appears in Collections:	[Department of Horticulture] journal articles

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	198	View/Open

View Licence

Loading...