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https://irlib.pccu.edu.tw/handle/987654321/41846
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| 題名: | Characterization of nitrate assimilation in Lactuca sativa L. under different nitrogen sources |
| 作者: | Zhong, FL (Zhong, Fenglin)
Wang, SB (Wang, Shubin)
Lin, JF (Lin, Junfang)
Roan, SF (Roan, Su-feng)
Lin, BY (Lin, Biying)
Zhou, XZ (Zhou, Xiangzhu)
Chen, IZ (Chen, Iou-zen)
Lin, YZ (Lin, Yizhang)
Pang, J (Pang, Jie)
Wu, S (Wu, Shuang) |
| 貢獻者: | 園藝暨生物技術學系 |
| 關鍵詞: | NITRITE REDUCTION
PLANTS
SPINACH
LETTUCE
GENES
RATIO |
| 日期: | 2018-09 |
| 上傳時間: | 2019-01-16 16:07:17 (UTC+8) |
| 摘要: | Optimal fertilization management is critical for romaine lettuce (Lactuca dolichophylla K.) cultivation, a major economical vegetable in China. Herein, we chose a romaine lettuce genotype (Lactuca sativa L.) known for "year-round production" and as "high-yielding," to examine the effect of varied nitrogen (N) fertilizer forms on nitrate (NO3-) accumulation. We cloned NO3- reductase (NR) and nitrite (NO2-) reductase (NiR) of romaine lettuce and analyzed their expressions at different growth stages in response to different N forms. Five N treatments with varying NO3--N:NH4+-N ratios were examined. Our results showed that lettuce leaf NO3- content decreased significantly with the increase of NH4+-N in fertilizer. The lowest NO3- content was observed when NO3--N:NH4+-N ratio was 0:100. NR and NiR activity and expression initially were upregulated by the increase in NO3- content, with the highest level at 70:30 NO3--N:NH4+-N ratio, but quickly declined thereafter. We also found the temporal activation pattern of N transporters was different from that of NO3- reductase and NO2- reductase, suggesting there is a coordination between N translocation and N reduction in lettuce leaves to maintain proper N homeostasis. These findings provide insights into the role of NO3--N:NH4+-N ratio in regulating NO3- assimilation, balancing N metabolism, and reducing NO2- toxicity in vegetable production. |
| 顯示於類別: | [園藝暨生物技術學系] 期刊論文
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