文化大學機構典藏 CCUR:Item 987654321/41846
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/41846


    题名: Characterization of nitrate assimilation in Lactuca sativa L. under different nitrogen sources
    作者: Zhong, FL (Zhong, Fenglin)
    Wang, SB (Wang, Shubin)
    Lin, JF (Lin, Junfang)
    Roan, SF (Roan, Su-feng)
    Lin, BY (Lin, Biying)
    Zhou, XZ (Zhou, Xiangzhu)
    Chen, IZ (Chen, Iou-zen)
    Lin, YZ (Lin, Yizhang)
    Pang, J (Pang, Jie)
    Wu, S (Wu, Shuang)
    贡献者: 園藝暨生物技術學系
    关键词: NITRITE REDUCTION
    PLANTS
    SPINACH
    LETTUCE
    GENES
    RATIO
    日期: 2018-09
    上传时间: 2019-01-16 16:07:17 (UTC+8)
    摘要: Optimal fertilization management is critical for romaine lettuce (Lactuca dolichophylla K.) cultivation, a major economical vegetable in China. Herein, we chose a romaine lettuce genotype (Lactuca sativa L.) known for "year-round production" and as "high-yielding," to examine the effect of varied nitrogen (N) fertilizer forms on nitrate (NO3-) accumulation. We cloned NO3- reductase (NR) and nitrite (NO2-) reductase (NiR) of romaine lettuce and analyzed their expressions at different growth stages in response to different N forms. Five N treatments with varying NO3--N:NH4+-N ratios were examined. Our results showed that lettuce leaf NO3- content decreased significantly with the increase of NH4+-N in fertilizer. The lowest NO3- content was observed when NO3--N:NH4+-N ratio was 0:100. NR and NiR activity and expression initially were upregulated by the increase in NO3- content, with the highest level at 70:30 NO3--N:NH4+-N ratio, but quickly declined thereafter. We also found the temporal activation pattern of N transporters was different from that of NO3- reductase and NO2- reductase, suggesting there is a coordination between N translocation and N reduction in lettuce leaves to maintain proper N homeostasis. These findings provide insights into the role of NO3--N:NH4+-N ratio in regulating NO3- assimilation, balancing N metabolism, and reducing NO2- toxicity in vegetable production.
    显示于类别:[園藝暨生物技術學系] 期刊論文

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