蛋白質超細纖維支架在成分和結構上具有類比細胞外基質的潛力，但往往水穩定性較差，在細胞培養環境中而難以維持超細纖維結構，因此需要進行改性處理。本研究針對植物蛋白、寡基蛋白二維靜電紡超細纖維體系以及三維相分離超細纖維體系的特點，開發基於檸檬酸的無毒交聯處理方法。研究證明，檸檬酸無毒交聯體系可有效提高蛋白質超細纖維支架的水穩定性，纖維支架具有良好的生物相適性，具有促進附著與增殖等細胞行為的功能。
Protein-based scaffolds have the potential to mimic the native extracellular matrices, but they suffer from the poor water stability, especially those with ultrafine fibrous structures. Therefore, modifications are required. In this research, the crosslinking modifications using citric acid as the crosslinker were developed based on the properties of 2D electrospun and 3D phase separated fibers from plant proteins or proteins with limited available functional groups. It was proven that the citric acid modifications could efficiently improve the water stability of the protein ultrafine fibrous scaffolds. Compared with the poly (lactic acid) scaffolds, these developed scaffolds were more cytocompatible and could promote cell attachment and proliferation.
