Simultaneous Analysis of Free/Bound Phytosterols and Diosgenin in Taiwanese Yams

文化大學機構典藏 CCUR > 環境規劃暨生物資源學院 > 農學院 > 生活應用科學系暨生活應用科學研究所 > 期刊論文 > Item 987654321/36132

Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/36132

Title:	Simultaneous Analysis of Free/Bound Phytosterols and Diosgenin in Taiwanese Yams
Authors:	Lu, TJ (Lu, Ting-Jang) Yu, CW (Yu, Chia-Wen) Lin, YC (Lin, Yu-Chih) Wang, HJ (Wang, Huei-Ju)
Contributors:	生應系
Keywords:	Phytosterols Diosgenin Dioscorea GC-FID HPLC-UV-ELSD
Date:	2017-05
Issue Date:	2017-06-08 10:20:38 (UTC+8)
Abstract:	Gas chromatography (GC) and high performance liquid chromatography (HPLC) methods were developed for the simultaneous analysis of phytosterols and diosgenin in two representative Taiwanese yams, Dioscorea pseudojaponica Yamamoto and Dioscorea alata L. The major phytosterols found were beta-sitosterol, stigmasterol, and campesterol. GC afforded good separation of phytosterols and diosgenin, with the diosgenin peak appearing between stigmasterol and beta-sitosterol. For HPLC, UV detection had higher sensitivity (detection limits 0.03-0.09 mu g); however, evaporative light scattering detection (ELSD) achieved higher noise tolerance and did not show baseline interference from solvent gradients. In addition, mixed solvents for extraction before acid hydrolysis, which is necessary to avoid fibers and acid hydrolysates binding to the target compounds, were evaluated. Phytosterol contents of 30.1 and 9.9 mg/100 g were found in D. pseudojaponica Yamamoto and D. alata L., respectively. Diosgenin was only found in D. pseudojaponica Yamamoto at a content of 15.2 mg/100 g.
Relation:	FOOD ANALYTICAL METHODS 卷: 10 期: 5 頁碼: 1172-1180
Appears in Collections:	[生活應用科學系暨生活應用科學研究所] 期刊論文

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	387	View/Open

View Licence

Loading...