Simultaneous Analysis of Free/Bound Phytosterols and Diosgenin in Taiwanese Yams

CCUR > College of Environmental Planning & Bioresources > College of Agriculture > Department of Applied Science of Living & Graduate Institute of Applied Science of Living > journal articles > Item 987654321/36132

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题名:	Simultaneous Analysis of Free/Bound Phytosterols and Diosgenin in Taiwanese Yams
作者:	Lu, TJ (Lu, Ting-Jang) Yu, CW (Yu, Chia-Wen) Lin, YC (Lin, Yu-Chih) Wang, HJ (Wang, Huei-Ju)
贡献者:	生應系
关键词:	Phytosterols Diosgenin Dioscorea GC-FID HPLC-UV-ELSD
日期:	2017-05
上传时间:	2017-06-08 10:20:38 (UTC+8)
摘要:	Gas chromatography (GC) and high performance liquid chromatography (HPLC) methods were developed for the simultaneous analysis of phytosterols and diosgenin in two representative Taiwanese yams, Dioscorea pseudojaponica Yamamoto and Dioscorea alata L. The major phytosterols found were beta-sitosterol, stigmasterol, and campesterol. GC afforded good separation of phytosterols and diosgenin, with the diosgenin peak appearing between stigmasterol and beta-sitosterol. For HPLC, UV detection had higher sensitivity (detection limits 0.03-0.09 mu g); however, evaporative light scattering detection (ELSD) achieved higher noise tolerance and did not show baseline interference from solvent gradients. In addition, mixed solvents for extraction before acid hydrolysis, which is necessary to avoid fibers and acid hydrolysates binding to the target compounds, were evaluated. Phytosterol contents of 30.1 and 9.9 mg/100 g were found in D. pseudojaponica Yamamoto and D. alata L., respectively. Diosgenin was only found in D. pseudojaponica Yamamoto at a content of 15.2 mg/100 g.
關聯:	FOOD ANALYTICAL METHODS 卷: 10 期: 5 頁碼: 1172-1180
显示于类别:	[Department of Applied Science of Living & Graduate Institute of Applied Science of Living ] journal articles

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