In this research, the polypropylene nonwoven fabric grafted with acrylic acid using benzoyl peroxide as a chemical initiator for the polymerization and then immobilized with a mixture of collagen and Rhizochitosan by glutaraldehyde as cross-linker had been investigated. The Rhizochitin was treated with different alkali, alkalinity, alkali-treated time, and treated-temperature for deacetylation. The experimental results showed that the degree of deacetylation increased with the increase of alkalinity, alkali-treated time and treated-temperature. But the Rhizochitosan's recovery decreased with the increase of alkalinity, alkali-treated time and temperature. The best degree of deacetylation and Rhizochitosan's recovery was got when the alkalinity 50%, alkali-treated 3 hours, treated-temperature 100℃. On the other hand, the swelling ratio decreases with the increase of the ratio of Rhizochitosan in the composites and the pH values in buffer solutions.