文化大學機構典藏 CCUR:Item 987654321/33314
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/33314


    题名: Endothelial Cell Loss Induced by Phacoemulsification Occurs Through Apoptosis
    超音波晶體乳化術後角膜內皮細胞的癒合機制是經由細胞凋亡過程所致
    作者: 賴麗如
    陳永豐
    吳賜猛
    曹友平
    蔡瑞芳
    贡献者: 生科系
    关键词: Apoptosis
    Corneal endothelium
    Phacoemulsification
    Ultrasound
    Cataract surgery
    In vivo rabbit model
    細胞凋亡
    角膜內皮細胞
    超音波晶體乳化術
    超音波
    白內障手術
    兔子動物實驗
    日期: 2001-10
    上传时间: 2016-07-27 10:25:28 (UTC+8)
    摘要: BACKGROUND: Cornea endothelial cells are nondividing cells containing pumping function which is crucial for cornea clarity and integrity. Endothelial cell loss occurs after cataract surgical procedures such as phacoemulsification. The authors hypothesize that endothelium damage occurs through apoptosis. METHODS: Ultrasound was achieved by placing a phacoemulsification probe in the anterior chamber and delivering 0% or 50% of maximum power for 2.5 min. The corneal tissue was harvested immediately, and at 1 and 7 days after the operation. Corneal tissue was stained by hemotoxylin and eosin (H&E) and evaluated by light microscopy. Endothelium apoptosis was monitored using the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick-end labeling (TUNEL) assay to detect DNA fragmentation. RESULTS: In corneas which underwent phacoemulsification with 0% energy, no cell loss or apoptosis was identified immediately, 1 or 7 days after the operation. Likewise, in corneas exposed to 50% energy, no cell loss or apoptosis was detected immediately after phacoemulsification. However, minimal amount of cell loss but prominent apoptosis was detected with the TUNEL assay 1 day after the operation, whereas significant cell loss but no apoptosis was detected 7 days after the operation by H & E stain. CONCLUSIONS: These results demonstrate that corneal endothelial cell loss induced by ultrasound damage occurs through apoptosis.
    關聯: 長庚醫學 24:10 民90.10 頁621-627
    显示于类别:[生命科學系] 期刊論文

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