Endothelial Cell Loss Induced by Phacoemulsification Occurs Through Apoptosis

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Title:	Endothelial Cell Loss Induced by Phacoemulsification Occurs Through Apoptosis 超音波晶體乳化術後角膜內皮細胞的癒合機制是經由細胞凋亡過程所致
Authors:	賴麗如陳永豐吳賜猛曹友平蔡瑞芳
Contributors:	生科系
Keywords:	Apoptosis Corneal endothelium Phacoemulsification Ultrasound Cataract surgery In vivo rabbit model 細胞凋亡角膜內皮細胞超音波晶體乳化術超音波白內障手術兔子動物實驗
Date:	2001-10
Issue Date:	2016-07-27 10:25:28 (UTC+8)
Abstract:	BACKGROUND: Cornea endothelial cells are nondividing cells containing pumping function which is crucial for cornea clarity and integrity. Endothelial cell loss occurs after cataract surgical procedures such as phacoemulsification. The authors hypothesize that endothelium damage occurs through apoptosis. METHODS: Ultrasound was achieved by placing a phacoemulsification probe in the anterior chamber and delivering 0% or 50% of maximum power for 2.5 min. The corneal tissue was harvested immediately, and at 1 and 7 days after the operation. Corneal tissue was stained by hemotoxylin and eosin (H&E) and evaluated by light microscopy. Endothelium apoptosis was monitored using the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick-end labeling (TUNEL) assay to detect DNA fragmentation. RESULTS: In corneas which underwent phacoemulsification with 0% energy, no cell loss or apoptosis was identified immediately, 1 or 7 days after the operation. Likewise, in corneas exposed to 50% energy, no cell loss or apoptosis was detected immediately after phacoemulsification. However, minimal amount of cell loss but prominent apoptosis was detected with the TUNEL assay 1 day after the operation, whereas significant cell loss but no apoptosis was detected 7 days after the operation by H & E stain. CONCLUSIONS: These results demonstrate that corneal endothelial cell loss induced by ultrasound damage occurs through apoptosis.
Relation:	長庚醫學 24:10 民90.10 頁621-627
Appears in Collections:	[Department of Biology ] journal articles

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