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    題名: New Insights into the Evolutionary Rate of Hepatitis B Virus at Different Biological Scales
    作者: Lin, You-Yu
    Liu, Chieh
    Chien, Wei-Hung
    Wu, Li-Ling
    Tao, Yong
    Wu, Dafei
    Lu, Xuemei
    Hsieh, Chia-Hung
    Chen, Pei-Jer
    Wang, Hurng-Yi
    Kao, Jia-Horng
    Chen, Ding-Shinn
    貢獻者: Dept Forestry & Nat Conservat
    關鍵詞: NATURAL-HISTORY
    RNA VIRUSES
    INFECTION
    HIV-1
    DISEASE
    HOST
    DYNAMICS
    MUTATION
    GENOME
    TRANSMISSION
    日期: 2015-04
    上傳時間: 2015-10-30 13:40:19 (UTC+8)
    摘要: The evolutionary rates of hepatitis B virus (HBV) estimated using contemporary sequences are 10(2) to 10(4) times higher than those derived from archaeological and genetic evidence. This discrepancy makes the origin of HBV and the time scale of its spread, both of which are critical for studying the burden of HBV pathogenicity, largely unresolved. To evaluate whether the dual demands (i.e., adaptation within hosts and colonization between hosts) of the viral life cycle affect this conundrum, the HBV quasispecies dynamics within and among hosts from a family consisting of a grandmother, her 5 children, and her 2 granddaughters, all of whom presumably acquired chronic HBV through mother-to-infant transmission, were examined by PCR cloning and next-generation sequencing methods. We found that the evolutionary rate of HBV between hosts was considerably lower than that within hosts. Moreover, the between-host substitution rates of HBV decreased as transmission numbers between individuals increased. Both observations were due primarily to changes at nonsynonymous rather than synonymous sites. There were significantly more multiple substitutions than expected for random mutation processes, and 97% of substitutions were changed from common to rare amino acid residues in the database. Continual switching between colonization and adaptation resulted in a rapid accumulation of mutations at a limited number of positions, which quickly became saturated, whereas substitutions at the remaining regions occurred at a much lower rate. Our study may help to explain the time-dependent HBV substitution rates reported in the literature and provide new insights into the origin of the virus.

    IMPORTANCE

    It is known that the estimated hepatitis B virus (HBV) substitution rate is time dependent, but the reason behind this observation is still elusive. We hypothesize that owing to the small genome size of HBV, transmission between hosts and adaptation within hosts must exhibit high levels of fitness trade-offs for the virus. By studying the HBV quasispecies dynamics for a chain of sequentially infected transmissions within a family, we found the HBV substitution rate between patients to be negatively correlated with the number of transmissions. Continual switching between hosts resulted in a rapid accumulation of mutations at a limited number of genomic sites, which quickly became saturated in the short term. Nevertheless, substitutions at the remaining regions occurred at a much lower rate. Therefore, the HBV substitution rate decreased as the divergence time increased.
    關聯: JOURNAL OF VIROLOGY 卷: 89 期: 7 頁碼: 3512-3522 DOI: 10.1128/JVI.03131-14
    顯示於類別:[森林暨自然保育學系 ] 期刊論文

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