数据加载中.....
|
jsp.display-item.identifier=請使用永久網址來引用或連結此文件:
https://irlib.pccu.edu.tw/handle/987654321/30754
|
| 题名: | New Insights into the Evolutionary Rate of Hepatitis B Virus at Different Biological Scales |
| 作者: | Lin, You-Yu
Liu, Chieh
Chien, Wei-Hung
Wu, Li-Ling
Tao, Yong
Wu, Dafei
Lu, Xuemei
Hsieh, Chia-Hung
Chen, Pei-Jer
Wang, Hurng-Yi
Kao, Jia-Horng
Chen, Ding-Shinn |
| 贡献者: | Dept Forestry & Nat Conservat |
| 关键词: | NATURAL-HISTORY
RNA VIRUSES
INFECTION
HIV-1
DISEASE
HOST
DYNAMICS
MUTATION
GENOME
TRANSMISSION |
| 日期: | 2015-04 |
| 上传时间: | 2015-10-30 13:40:19 (UTC+8) |
| 摘要: | The evolutionary rates of hepatitis B virus (HBV) estimated using contemporary sequences are 10(2) to 10(4) times higher than those derived from archaeological and genetic evidence. This discrepancy makes the origin of HBV and the time scale of its spread, both of which are critical for studying the burden of HBV pathogenicity, largely unresolved. To evaluate whether the dual demands (i.e., adaptation within hosts and colonization between hosts) of the viral life cycle affect this conundrum, the HBV quasispecies dynamics within and among hosts from a family consisting of a grandmother, her 5 children, and her 2 granddaughters, all of whom presumably acquired chronic HBV through mother-to-infant transmission, were examined by PCR cloning and next-generation sequencing methods. We found that the evolutionary rate of HBV between hosts was considerably lower than that within hosts. Moreover, the between-host substitution rates of HBV decreased as transmission numbers between individuals increased. Both observations were due primarily to changes at nonsynonymous rather than synonymous sites. There were significantly more multiple substitutions than expected for random mutation processes, and 97% of substitutions were changed from common to rare amino acid residues in the database. Continual switching between colonization and adaptation resulted in a rapid accumulation of mutations at a limited number of positions, which quickly became saturated, whereas substitutions at the remaining regions occurred at a much lower rate. Our study may help to explain the time-dependent HBV substitution rates reported in the literature and provide new insights into the origin of the virus.
IMPORTANCE
It is known that the estimated hepatitis B virus (HBV) substitution rate is time dependent, but the reason behind this observation is still elusive. We hypothesize that owing to the small genome size of HBV, transmission between hosts and adaptation within hosts must exhibit high levels of fitness trade-offs for the virus. By studying the HBV quasispecies dynamics for a chain of sequentially infected transmissions within a family, we found the HBV substitution rate between patients to be negatively correlated with the number of transmissions. Continual switching between hosts resulted in a rapid accumulation of mutations at a limited number of genomic sites, which quickly became saturated in the short term. Nevertheless, substitutions at the remaining regions occurred at a much lower rate. Therefore, the HBV substitution rate decreased as the divergence time increased. |
| 關聯: | JOURNAL OF VIROLOGY 卷: 89 期: 7 頁碼: 3512-3522 DOI: 10.1128/JVI.03131-14 |
| 显示于类别: | [Department of Forestry and Nature Conservation] journal articles
|
文件中的档案:
| 档案 |
描述 |
大小 | 格式 | 浏览次数 |
|---|
| index.html | | 0Kb | HTML | 464 | 检视/开启 |
|
在CCUR中所有的数据项都受到原著作权保护.
|
