已證明了哥王(Wikstroemia indica)，含有豐富的生物活性成分，用於治療許多疾病，如梅毒、關節炎、百日咳和癌症。利用細胞培養方法可用於擴增 MCF-7 的菌數並檢測了哥王提取物的抗腫瘤作用。
我們將了哥王使用三種不同的標準萃取其化合物，之後通過細胞增殖(MTT)、細胞遷移、細胞週期、細胞凋亡及 real-time RT-PCR 進行檢測。首先，將在四個不同時間及不同濃度的了哥王萃取液，加入人類乳腺癌細胞MCF-7 中，我們用 MTT 確定當濃度增加時其具有抗 MCF-7 增殖的能力。
了哥王的三種萃取液(樣品 A, B, C)在最高濃度中顯著抑制細胞增殖的結果。除樣品 A 外，當萃取液加入 MCF-7 六小時後其能有效抑制 MCF-7 二十四小時內的增殖。其次，相同濃度（ 130 微克/毫升）在細胞遷移中有類似的抑制效果，可抑制十二小時。第三，在細胞週期的結果中，我們預計哥王提取物主要捕捉到 MCF-7 細胞的週期為亞 G1 期，通過細胞凋亡試驗，得知細胞亞 G1 週期與細胞凋亡程序有關，但在 real-time PCR 中並沒有相應的結果，經前人研究指出有另一種細胞凋亡機制。
因此，希望實驗中顯著的結果，可以在乳腺癌的治療中作為有用抗癌藥物的證據及辦法。
Wikstroemia indica has been demonstrated as a plant that contains the most abundant bioactivity components for treating many diseases as syphilis, arthritis, whooping cough, and cancer. Cell culture methods can be applied to amplify MCF-7 cell populations and detect the antitumor effects of W. indica extracts. We assess 3 differently extractive criteria of W. indica compounds via cell-proliferation (MTT), cell-migration, cell cycle, apoptosis and real-time – reverse transcriptase – polymerase chain reaction (real-time RT-PCR) assays.
Initially, we determined its anticancer potential at increasingly serial concentrations from 8.125 to 130 µg/ml of the W. indica extracts with 4 different time frames. W. indica extracts remarkably inhibited cell proliferation result at highest concentrations of 3 samples (Sample A, B, C) after 6 hours hence constantly lasting inhibition ability for 24 hours. Secondly, migration results have similar effect with the same concentration (130 µg/ml) after 12h. Thirdly, cell cycle result as we expected that W. indica extracts to
trap major of MCF-7 cells in Sub-G1 phase. Due to sub-G1 phase ambushed cells related to cell death program via apoptosis assay. The real-time RT-PCR results could convince the previous experiments to discover the intracellular apoptotic mechanism.
Hopefully, these significant results could improve evidences for useful anticancer agents hence approach in breast cancer treatments.
