南嶺蕘花萃取物抗乳癌效果之研究

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Title:	南嶺蕘花萃取物抗乳癌效果之研究 Antitumor Effects of Wikstroemia indica Extracts on Human Breast Cancer MCF-7 Cells
Authors:	裴氏玉孝
Contributors:	生物科技研究所
Keywords:	MCF-7 細胞乳腺癌了哥王細胞增殖抗腫瘤抗遷移 real-time RT-PCR MCF-7 cells breast cancer Wikstroemia indica cell proliferation antitumor migration real-time RT-PCR
Date:	2015-06
Issue Date:	2015-08-19 13:37:32 (UTC+8)
Abstract:	已證明了哥王(Wikstroemia indica)，含有豐富的生物活性成分，用於治療許多疾病，如梅毒、關節炎、百日咳和癌症。利用細胞培養方法可用於擴增 MCF-7 的菌數並檢測了哥王提取物的抗腫瘤作用。我們將了哥王使用三種不同的標準萃取其化合物，之後通過細胞增殖(MTT)、細胞遷移、細胞週期、細胞凋亡及 real-time RT-PCR 進行檢測。首先，將在四個不同時間及不同濃度的了哥王萃取液，加入人類乳腺癌細胞MCF-7 中，我們用 MTT 確定當濃度增加時其具有抗 MCF-7 增殖的能力。了哥王的三種萃取液(樣品 A, B, C)在最高濃度中顯著抑制細胞增殖的結果。除樣品 A 外，當萃取液加入 MCF-7 六小時後其能有效抑制 MCF-7 二十四小時內的增殖。其次，相同濃度（ 130 微克/毫升）在細胞遷移中有類似的抑制效果，可抑制十二小時。第三，在細胞週期的結果中，我們預計哥王提取物主要捕捉到 MCF-7 細胞的週期為亞 G1 期，通過細胞凋亡試驗，得知細胞亞 G1 週期與細胞凋亡程序有關，但在 real-time PCR 中並沒有相應的結果，經前人研究指出有另一種細胞凋亡機制。因此，希望實驗中顯著的結果，可以在乳腺癌的治療中作為有用抗癌藥物的證據及辦法。 Wikstroemia indica has been demonstrated as a plant that contains the most abundant bioactivity components for treating many diseases as syphilis, arthritis, whooping cough, and cancer. Cell culture methods can be applied to amplify MCF-7 cell populations and detect the antitumor effects of W. indica extracts. We assess 3 differently extractive criteria of W. indica compounds via cell-proliferation (MTT), cell-migration, cell cycle, apoptosis and real-time – reverse transcriptase – polymerase chain reaction (real-time RT-PCR) assays. Initially, we determined its anticancer potential at increasingly serial concentrations from 8.125 to 130 µg/ml of the W. indica extracts with 4 different time frames. W. indica extracts remarkably inhibited cell proliferation result at highest concentrations of 3 samples (Sample A, B, C) after 6 hours hence constantly lasting inhibition ability for 24 hours. Secondly, migration results have similar effect with the same concentration (130 µg/ml) after 12h. Thirdly, cell cycle result as we expected that W. indica extracts to trap major of MCF-7 cells in Sub-G1 phase. Due to sub-G1 phase ambushed cells related to cell death program via apoptosis assay. The real-time RT-PCR results could convince the previous experiments to discover the intracellular apoptotic mechanism. Hopefully, these significant results could improve evidences for useful anticancer agents hence approach in breast cancer treatments.
Appears in Collections:	[Graduate Institute of Biotechnology ] thesis

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