| 题名: | Effect of caffeic acid phenethyl ester, an antioxidant from propolis, on inducing apoptosis in human leukemic HL-60 cells |
| 作者: | Chen YJ
Shiao MS
Hsu ML
Tsai HT
Wang SY |
| 贡献者: | 運動教練所 |
| 关键词: | caffeic acid phenethyl ester (CAPE)
apoptosis
caspase-3
Bcl-2
Bax |
| 日期: | 2001 |
| 上传时间: | 2009-12-11 11:52:34 (UTC+8) |
| 摘要: | Caffeic acid phenethyl ester (CAPE) is an active component isolated from propolis, The aim of this study was to investigate the mechanism of CAPE-induced apoptosis in human leukemic HL-60 cells. It was found that CAPE entered HL-60 cells very quickly and then inhibited their survival in a concentration- and time-dependent manner. CAPE induced characteristic DNA fragmentation and morphological changes typical of apoptosis in these cells. Estimation of the apoptotic percentage showed a time-dependent increase after CAPE (6 mug/mL) treatment (up to 66.7 +/- 2.0% at 72 h). Treatment with CAPE caused rapid activation of caspase-3 after 4 h, down-regulation of Bcl-2 expression after 6 h, and up-regulation of Bax expression after 16 h. These results suggest that CAPE is a potent apoptosis-inducing agent; its action is accompanied by activation of caspase-3, down-regulation of Bcl-2, and up-regulation of Bax in human leukemic HL-60 cells. |
| 關聯: | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Volume: 49 Issue: 11 Pages: 5615-5619 |
| 显示于类别: | [運動教練研究所] 期刊論文
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