Effect of caffeic acid phenethyl ester, an antioxidant from propolis, on inducing apoptosis in human leukemic HL-60 cells

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Title:	Effect of caffeic acid phenethyl ester, an antioxidant from propolis, on inducing apoptosis in human leukemic HL-60 cells
Authors:	Chen YJ Shiao MS Hsu ML Tsai HT Wang SY
Contributors:	運動教練所
Keywords:	caffeic acid phenethyl ester (CAPE) apoptosis caspase-3 Bcl-2 Bax
Date:	2001
Issue Date:	2009-12-11 11:52:34 (UTC+8)
Abstract:	Caffeic acid phenethyl ester (CAPE) is an active component isolated from propolis, The aim of this study was to investigate the mechanism of CAPE-induced apoptosis in human leukemic HL-60 cells. It was found that CAPE entered HL-60 cells very quickly and then inhibited their survival in a concentration- and time-dependent manner. CAPE induced characteristic DNA fragmentation and morphological changes typical of apoptosis in these cells. Estimation of the apoptotic percentage showed a time-dependent increase after CAPE (6 mug/mL) treatment (up to 66.7 +/- 2.0% at 72 h). Treatment with CAPE caused rapid activation of caspase-3 after 4 h, down-regulation of Bcl-2 expression after 6 h, and up-regulation of Bax expression after 16 h. These results suggest that CAPE is a potent apoptosis-inducing agent; its action is accompanied by activation of caspase-3, down-regulation of Bcl-2, and up-regulation of Bax in human leukemic HL-60 cells.
Relation:	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Volume: 49 Issue: 11 Pages: 5615-5619
Appears in Collections:	[Graduate Institute of Sport Coaching Science ] journal articles

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