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請使用永久網址來引用或連結此文件:
https://irlib.pccu.edu.tw/handle/987654321/29260
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| 題名: | Concentration Variation and Molecular Characteristics of Soluble (1,3;1,6)-beta-D-Glucans in Submerged Cultivation Products of Ganoderma lucidum Mycelium |
| 作者: | Wang, Chung-Huang
Hsieh, Shu-Chen
Wang, Huei-Ju
Chen, Miaw-Ling
Lin, Bi-Fong
Chiang, Been-Huang
Lu, Ting-Jang |
| 貢獻者: | 生活應用科學系 |
| 關鍵詞: | 0,3,1,043-beta-glucans
Ganoderma lucidum
degree of branching
molecular weight |
| 日期: | 2014-01-22 |
| 上傳時間: | 2015-01-27 14:28:06 (UTC+8) |
| 摘要: | (1,3)-fi-beta-Glucans with (1,6)-f3-beta-glucosyl branches are bioactive polysaccharides in fruiting bodies and mycelia of Ganoderma lucidum, a mushroom used in traditional Chinese medicine. Submerged cultivation of mycelium is one of the more efficient means of generating polysaccharides from this fungus. Twelve mycelium samples examined in this study demonstrated the quantitative and qualitative molecular characteristics of soluble (1,3;1,6)-fi-beta-glucans. It was observed that the concentration of soluble (1,3;1,6)-fl-beta-glucan varied substantially from 1.3 to 79.9 mg/dL. (1,3;1,6)-j9-beta-Glucans also preserved their molecular characteristics with degrees of branching (DB) of 0.21-0.36 and molecular masses of 105-106 g/mol for those samples with substantial quantities of /3-D-glucan. Using the high aggregating tendency of these molecules, (1,3;1,6)-fl-beta-glucans were successfully purified via fractional precipitation with 35% (v/v) ethanol. (1,3;1,6)-fl-beta-Glucan was proposed as a putative bioactive marker for immunomodulation because it was the most abundant polysaccharide in G. lucidum mycelium products to stimulate macrophage RAW 264.7 cells to release TNF-alpha. |
| 關聯: | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 卷: 62 期: 3 頁碼: 634-641 |
| 顯示於類別: | [生活應用科學系暨生活應用科學研究所] 期刊論文
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