Concentration Variation and Molecular Characteristics of Soluble (1,3;1,6)-beta-D-Glucans in Submerged Cultivation Products of Ganoderma lucidum Mycelium

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Title:	Concentration Variation and Molecular Characteristics of Soluble (1,3;1,6)-beta-D-Glucans in Submerged Cultivation Products of Ganoderma lucidum Mycelium
Authors:	Wang, Chung-Huang Hsieh, Shu-Chen Wang, Huei-Ju Chen, Miaw-Ling Lin, Bi-Fong Chiang, Been-Huang Lu, Ting-Jang
Contributors:	生活應用科學系
Keywords:	0,3,1,043-beta-glucans Ganoderma lucidum degree of branching molecular weight
Date:	2014-01-22
Issue Date:	2015-01-27 14:28:06 (UTC+8)
Abstract:	(1,3)-fi-beta-Glucans with (1,6)-f3-beta-glucosyl branches are bioactive polysaccharides in fruiting bodies and mycelia of Ganoderma lucidum, a mushroom used in traditional Chinese medicine. Submerged cultivation of mycelium is one of the more efficient means of generating polysaccharides from this fungus. Twelve mycelium samples examined in this study demonstrated the quantitative and qualitative molecular characteristics of soluble (1,3;1,6)-fi-beta-glucans. It was observed that the concentration of soluble (1,3;1,6)-fl-beta-glucan varied substantially from 1.3 to 79.9 mg/dL. (1,3;1,6)-j9-beta-Glucans also preserved their molecular characteristics with degrees of branching (DB) of 0.21-0.36 and molecular masses of 105-106 g/mol for those samples with substantial quantities of /3-D-glucan. Using the high aggregating tendency of these molecules, (1,3;1,6)-fl-beta-glucans were successfully purified via fractional precipitation with 35% (v/v) ethanol. (1,3;1,6)-fl-beta-Glucan was proposed as a putative bioactive marker for immunomodulation because it was the most abundant polysaccharide in G. lucidum mycelium products to stimulate macrophage RAW 264.7 cells to release TNF-alpha.
Relation:	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 卷: 62 期: 3 頁碼: 634-641
Appears in Collections:	[Department of Applied Science of Living & Graduate Institute of Applied Science of Living ] journal articles

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