| 題名: | Uncoupling of promoter methylation and expression of Period1 in cervical cancer cells |
| 作者: | Hsu, Min-Chuan
Huang, Chih-Cheng
Choo, Kong-Bung
Huang, Chiu-Jung |
| 貢獻者: | 動科系 |
| 關鍵詞: | cervical cancer
CpG islands
E-box
methylation analysis
Period1 gene
promoter methylation
transcription factors
transcriptional regulation |
| 日期: | 2007 |
| 上傳時間: | 2009-11-13 11:28:33 (UTC+8) |
| 摘要: | We investigated possible epigenetic regulation of Period1 (PER1), a key circadian regulator gene, in six cervical cancer cell lines which showed up to 15.4-fold differences in PER1 mRNA levels. Genomic methylation analysis showed that a discerned CpG island in the PERI promoter remained hypomethylated in five of the cell lines. In contrast, C33A cells that showed maximal PER1 expression was hypermethylated; however, demethylation treatment of C33A cells resulted in small but significant elevated PERI mRNA levels suggesting a secondary role for promoter hypermethylation in PER1 transcriptional regulation. A discerned hypomethylated zone that harbours crucial transcriptional elements including the critical proximal E-box progressively diminished in size in the cell lines until a methylation-resistant core was retained in C33A. Our data indicate that PER1 transcription is mainly uncoupled from promoter methylation but probably involves availability and interactions of trans-acting factors with differentially methylated cis elements in the promoter hypomethylated zone. (c) 2007 Elsevier Inc. All rights reserved. |
| 關聯: | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 360 Issue: 1 Pages: 257-262 |
| 顯示於類別: | [動物科學系 ] 期刊論文
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