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    Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/2661


    Title: Uncoupling of promoter methylation and expression of Period1 in cervical cancer cells
    Authors: Hsu, Min-Chuan
    Huang, Chih-Cheng
    Choo, Kong-Bung
    Huang, Chiu-Jung
    Contributors: 動科系
    Keywords: cervical cancer
    CpG islands
    E-box
    methylation analysis
    Period1 gene
    promoter methylation
    transcription factors
    transcriptional regulation
    Date: 2007
    Issue Date: 2009-11-13 11:28:33 (UTC+8)
    Abstract: We investigated possible epigenetic regulation of Period1 (PER1), a key circadian regulator gene, in six cervical cancer cell lines which showed up to 15.4-fold differences in PER1 mRNA levels. Genomic methylation analysis showed that a discerned CpG island in the PERI promoter remained hypomethylated in five of the cell lines. In contrast, C33A cells that showed maximal PER1 expression was hypermethylated; however, demethylation treatment of C33A cells resulted in small but significant elevated PERI mRNA levels suggesting a secondary role for promoter hypermethylation in PER1 transcriptional regulation. A discerned hypomethylated zone that harbours crucial transcriptional elements including the critical proximal E-box progressively diminished in size in the cell lines until a methylation-resistant core was retained in C33A. Our data indicate that PER1 transcription is mainly uncoupled from promoter methylation but probably involves availability and interactions of trans-acting factors with differentially methylated cis elements in the promoter hypomethylated zone. (c) 2007 Elsevier Inc. All rights reserved.
    Relation: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 360 Issue: 1 Pages: 257-262
    Appears in Collections:[Department of Animal Science] journal articles

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