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| Title: | Effect of cultivation conditions on spore production from Bacillus amyloliquefaciens B128 and its antagonism to Botrytis elliptica |
| Authors: | Tzeng, Y. -M.
Rao, Y. K.
Tsay, K. -J.
Wu, W. -S. |
| Contributors: | 園生系 |
| Keywords: | airlift bioreactor
antagonism
Bacillus amyloliquefaciens
Botrytis elliptica
spore production |
| Date: | 2008 |
| Issue Date: | 2009-11-06 14:39:59 (UTC+8) |
| Abstract: | Aims: To maximize spore production by Bacillus amyloliquefaciens B128, and its antagonism to the fungal pathogen Botrytis elliptica B061.
Methods and Results: In the 5-l stirred-tank bioreactor (STR), with the 0.5 vvm aeration rate, an agitation rate of 200 rev min(-1) significantly enhanced the spore yield compared to the same in 300 rev min(-1) cultivations. In a 20-l airlift bioreactor (ALR) the maximal spore production was further increased with a controlled aeration rate of 2.5 vvm operated in a 24-mesh net-draft tube mode, and no pH control cultivation. This spore yield in the 20-l ALR was five- and eightfold higher; in addition the cultivation period was 19 h shorter, compared to that obtained from shaker flask and in the 5-l STR cultivations respectively.
Conclusions: Although culture conditions are still to be optimized, by using an ALR with net-draft tube, a scaling up from shaker flasks and STR to ALR of spore production by the strain B128 is technically feasible.
Significance and Impact of the Study: The spore yields obtained using bioreactors were much higher than those previously reported. The freshly produced spore preparations from the B128 strain significantly antagonized the grey mould pathogen B. elliptica. |
| Relation: | JOURNAL OF APPLIED MICROBIOLOGY Volume: 104 Issue: 5 Pages: 1275-1282 |
| Appears in Collections: | [園藝暨生物技術學系] 期刊論文
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