Effect of cultivation conditions on spore production from Bacillus amyloliquefaciens B128 and its antagonism to Botrytis elliptica

文化大學機構典藏 CCUR > 環境規劃暨生物資源學院 > 農學院 > 園藝暨生物技術學系 > 期刊論文 > Item 987654321/2570

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题名:	Effect of cultivation conditions on spore production from Bacillus amyloliquefaciens B128 and its antagonism to Botrytis elliptica
作者:	Tzeng, Y. -M. Rao, Y. K. Tsay, K. -J. Wu, W. -S.
贡献者:	園生系
关键词:	airlift bioreactor antagonism Bacillus amyloliquefaciens Botrytis elliptica spore production
日期:	2008
上传时间:	2009-11-06 14:39:59 (UTC+8)
摘要:	Aims: To maximize spore production by Bacillus amyloliquefaciens B128, and its antagonism to the fungal pathogen Botrytis elliptica B061. Methods and Results: In the 5-l stirred-tank bioreactor (STR), with the 0.5 vvm aeration rate, an agitation rate of 200 rev min(-1) significantly enhanced the spore yield compared to the same in 300 rev min(-1) cultivations. In a 20-l airlift bioreactor (ALR) the maximal spore production was further increased with a controlled aeration rate of 2.5 vvm operated in a 24-mesh net-draft tube mode, and no pH control cultivation. This spore yield in the 20-l ALR was five- and eightfold higher; in addition the cultivation period was 19 h shorter, compared to that obtained from shaker flask and in the 5-l STR cultivations respectively. Conclusions: Although culture conditions are still to be optimized, by using an ALR with net-draft tube, a scaling up from shaker flasks and STR to ALR of spore production by the strain B128 is technically feasible. Significance and Impact of the Study: The spore yields obtained using bioreactors were much higher than those previously reported. The freshly produced spore preparations from the B128 strain significantly antagonized the grey mould pathogen B. elliptica.
關聯:	JOURNAL OF APPLIED MICROBIOLOGY Volume: 104 Issue: 5 Pages: 1275-1282
显示于类别:	[園藝暨生物技術學系] 期刊論文

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