白花海芋 (<I>Zantedeschia aethiopica </I>L.) 是陽明山極具重要性的經濟花卉作物,現今因種球老化及氣候異常的影響,又再度引發細菌性軟腐病的危害。為促進海芋產業的永續發展,在前期計畫中已建立由直接芽體器官發生的海芋試管內植株再生途徑,接續在本研究計畫將以培育及生產健康海芋種球為主要目標。本二年期的計畫中,將量化生產白花海芋芽體及種苗,以做為種球形成的植物材料。評估植株大小及液體或固體的培養方式,對試管內種球形成的影響,以確定最適養球的條件。接續將探討不同濃度的蔗糖與茉莉酸組合對種球發育及增殖的影響,以篩選出促進種球形成的關鍵要素及種球發展為開花球的基本要件。未來將可應用於開發白花海芋量產健康根莖種球的技術平台,並可供應農民健康種球更新的來源。
White calla lily ( <EM>Zantedeschia aethiopica</EM> L.) is an important economic flower crop in Yang-Ming-Shan National Park. Nowadays, soft rot disease causes serious production loss of calla lily again due to bulblet aging and climate abnormality. For sustainable development of calla lily industry, <I>in vitro</I> plant regeneration pathway of white calla lily through direct shoot organogenesis has been established in previous research. The objective of this study is to develop mass production system of healthy shoots and plantlets for further plant materials of bulblet formation . Different size of plantlets in liquid or solid culture will be estimated for the effects on bulblet formation. The combination of different concentrations of sucrose and jasmonic acid will be used to find out a suitable condition for bulblet development and proliferation. The development of bulblet to flowering bulb will be also discussed. The mass propagation of healthy rhizome bulb of white calla lily could serve as plant material source for growers to renew bulbs.