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https://irlib.pccu.edu.tw/handle/987654321/22928
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題名: | Alterations of biochemical parameters in malformed Indian rice frogs, Rana limnocharis from Southern Taiwan |
作者: | Chiu, Yuh-Wen Wang, Shu-Yin Wu, Jui-Pin Huang, Da-Ji |
貢獻者: | 動物科學系 |
關鍵詞: | INDUCTION COLORIMETRIC DETERMINATION XENOPUS-TROPICALIS MICROPLATE ASSAY MALFORMATIONS AMPHIBIANS EXPOSURE METHOXYCHLOR GLUTATHIONE RESISTANCE |
日期: | 2011-11 |
上傳時間: | 2012-09-06 14:01:31 (UTC+8) |
摘要: | The purpose of this study is to investigate the factors that cause malformed frogs in upstream Kaoping river (KP site) and Tungkang river (T site) of Southern Taiwan. In this experiment, the activities of monooxygenase (MO), glutathione-S-transferase (GST), acetylcholinesterase (AchE) as well as the concentration of vitellogenin (Vg) in the liver were measured. Results show that activities of MO, GST and AchE, and Vg levels in normal frogs (male/female) were 0.09 +/- 0.02/0.09 +/- 0.01 Delta A min(-1) mg(-1) protein, 0.12 +/- 0.04/0.13 +/- 0.04 Delta A min(-1) mg(-1) protein, 6.13 +/- 2.69/6.01 +/- 2.09 U mg(-1) protein and 0.87 +/- 0.42/2.18 +/- 0.50 mu g mg(-1) protein, respectively. Activities of MO, GST and AchE, and Vg levels in malformed frogs (male/female) were 0.15 +/- 0.04/0.21 +/- 0.07 Delta A min(-1) mg(-1) protein, 0.27 +/- 0.08/0.30 +/- 0.12 Delta A min(-1)mg(-1) protein, 4.59 +/- 2.71/5.19 +/- 3.74 U mg(-1) protein and 1.46 +/- 0.61/3.15 +/- 0.88 mu g mg(-1) protein, respectively in KP site, and were 0.16 +/- 0.69/0.18 +/- 0.07 Delta A min(-1) mg(-1) protein, 0.21 +/- 0.07/0.24 +/- 0.08,Delta A min(-1) mg(-1) protein, 5.13 +/- 4.58/3.94 +/- 1.33 U mg(-1) protein and 2.23 +/- 1.47/4.11 +/- 1.63 mu g mg(-1) protein, respectively in T site. These results indicate that male and female malformed frogs in both rivers upstream are found with higher activities. No significant difference in AchE activity was found between normal and malformed frogs in this investigation. It is therefore reasonable to speculate that the organic chemicals released from agricultural activities are presumable the main factors that lead to the malformation of frogs. |
關聯: | Source: JOURNAL OF ENVIRONMENTAL BIOLOGY Volume: 32 Issue: 6 Pages: 807-812 |
顯示於類別: | [動物科學系 ] 期刊論文
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