文化大學機構典藏 CCUR:Item 987654321/22928
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    题名: Alterations of biochemical parameters in malformed Indian rice frogs, Rana limnocharis from Southern Taiwan
    作者: Chiu, Yuh-Wen
    Wang, Shu-Yin
    Wu, Jui-Pin
    Huang, Da-Ji
    贡献者: 動物科學系
    关键词: INDUCTION
    COLORIMETRIC DETERMINATION
    XENOPUS-TROPICALIS
    MICROPLATE ASSAY
    MALFORMATIONS
    AMPHIBIANS
    EXPOSURE
    METHOXYCHLOR
    GLUTATHIONE
    RESISTANCE
    日期: 2011-11
    上传时间: 2012-09-06 14:01:31 (UTC+8)
    摘要: The purpose of this study is to investigate the factors that cause malformed frogs in upstream Kaoping river (KP site) and Tungkang river (T site) of Southern Taiwan. In this experiment, the activities of monooxygenase (MO), glutathione-S-transferase (GST), acetylcholinesterase (AchE) as well as the concentration of vitellogenin (Vg) in the liver were measured. Results show that activities of MO, GST and AchE, and Vg levels in normal frogs (male/female) were 0.09 +/- 0.02/0.09 +/- 0.01 Delta A min(-1) mg(-1) protein, 0.12 +/- 0.04/0.13 +/- 0.04 Delta A min(-1) mg(-1) protein, 6.13 +/- 2.69/6.01 +/- 2.09 U mg(-1) protein and 0.87 +/- 0.42/2.18 +/- 0.50 mu g mg(-1) protein, respectively. Activities of MO, GST and AchE, and Vg levels in malformed frogs (male/female) were 0.15 +/- 0.04/0.21 +/- 0.07 Delta A min(-1) mg(-1) protein, 0.27 +/- 0.08/0.30 +/- 0.12 Delta A min(-1)mg(-1) protein, 4.59 +/- 2.71/5.19 +/- 3.74 U mg(-1) protein and 1.46 +/- 0.61/3.15 +/- 0.88 mu g mg(-1) protein, respectively in KP site, and were 0.16 +/- 0.69/0.18 +/- 0.07 Delta A min(-1) mg(-1) protein, 0.21 +/- 0.07/0.24 +/- 0.08,Delta A min(-1) mg(-1) protein, 5.13 +/- 4.58/3.94 +/- 1.33 U mg(-1) protein and 2.23 +/- 1.47/4.11 +/- 1.63 mu g mg(-1) protein, respectively in T site. These results indicate that male and female malformed frogs in both rivers upstream are found with higher activities. No significant difference in AchE activity was found between normal and malformed frogs in this investigation. It is therefore reasonable to speculate that the organic chemicals released from agricultural activities are presumable the main factors that lead to the malformation of frogs.
    關聯: Source: JOURNAL OF ENVIRONMENTAL BIOLOGY Volume: 32 Issue: 6 Pages: 807-812
    显示于类别:[動物科學系 ] 期刊論文

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