This research studies the morphology and characterization of three-dimensional (3D) micro-porous structures produced from biodegradable chitosan for use as scaffolds for cells culture. The chitosan 3D micro-porous structures were produced by a simple liquid hardening method, which includes the processes of foaming by mechanical stirring without any chemical foaming agent added, and hardening by NaOH cross linking. The pore size and porosity were controlled with mechanical stirring strength. The scanning electronic microscopy (SEM) results showed that chitosan scaffold obtained by 4000 & 6000 rpm had the appropriate roughness for fibroblast attachment comparing with chitosan scaffold obtained by 2000 rpm.
