文化大學機構典藏 CCUR:Item 987654321/21017
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 46962/50828 (92%)
造访人次 : 12458958      在线人数 : 607
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于CCUR ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/21017


    题名: Ethephon-mediated effects on leaf senescence are affected by reduced glutathione and EGTA in sweet potato detached leaves
    作者: Chen, HJ (Chen, Hsien-Jung)
    Tsai, YJ (Tsai, Yi-Jing)
    Chen, WS (Chen, Wei-Shan)
    Huang, GJ (Huang, Guan-Jhong)
    Huang, SS (Huang, Shyh-Shyun)
    Lin, YH (Lin, Yaw-Huei)
    贡献者: 生科系
    关键词: CV TAINONG 57
    MOLECULAR-CLONING
    HYDROGEN-PEROXIDE
    IN-VITRO
    TRYPSIN-INHIBITOR
    ANTIOXIDANT ACTIVITIES
    IPOMOELIN GENE
    STORAGE ROOTS
    CELL-DEATH
    ETHYLENE
    日期: 2010-12
    上传时间: 2011-12-12 14:16:10 (UTC+8)
    摘要: In this report several senescence-associated markers were used to study the ethephon-mediated effects on leaf senescence in detached sweet potato leaves. The chlorophyll contents and Fv/Fm values were drastically reduced, however, H(2)O(2) contents detected with diaminobenzidine (DAB) staining and a papain-like cysteine protease SPCP1 expression were significantly enhanced in ethephon-treated leaves compared to untreated dark control. In the presence of reduced glutathione, EGTA or cycloheximide, the reduction of chlorophyll contents and Fv/Fm values were alleviated, however, the induction or enhancement of H(2)O(2) contents and cysteine protease SPCP1 expression were repressed. Both calcium ionophore A23187 and glutathione synthase inhibitor, L-buthionine sulfoximide (BSO), remarkably induced SPCP1 expression in detached leaves, and the induction was also repressed by EGTA and reduced glutathione, respectively. The time effective for cycloheximide repression of SPCP1 expression was ca. 6 to 12 hours after ethephon treatment. In conclusion, ethephon-mediated effects on leaf senescence and gene expression in detached sweet potato leaves are significantly repressed by reduced glutathione, EGTA, and cycloheximide, respectively. These data suggest a possible involvement of oxidative stress, external calcium influx, and de novo synthesized proteins in association with ethephon signaling leading to leaf senescence and gene expression in sweet potato detached leaves.
    显示于类别:[生命科學系] 期刊論文

    文件中的档案:

    没有与此文件相关的档案.



    在CCUR中所有的数据项都受到原著作权保护.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈