Ethephon-mediated effects on leaf senescence are affected by reduced glutathione and EGTA in sweet potato detached leaves

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Title:	Ethephon-mediated effects on leaf senescence are affected by reduced glutathione and EGTA in sweet potato detached leaves
Authors:	Chen, HJ (Chen, Hsien-Jung) Tsai, YJ (Tsai, Yi-Jing) Chen, WS (Chen, Wei-Shan) Huang, GJ (Huang, Guan-Jhong) Huang, SS (Huang, Shyh-Shyun) Lin, YH (Lin, Yaw-Huei)
Contributors:	生科系
Keywords:	CV TAINONG 57 MOLECULAR-CLONING HYDROGEN-PEROXIDE IN-VITRO TRYPSIN-INHIBITOR ANTIOXIDANT ACTIVITIES IPOMOELIN GENE STORAGE ROOTS CELL-DEATH ETHYLENE
Date:	2010-12
Issue Date:	2011-12-12 14:16:10 (UTC+8)
Abstract:	In this report several senescence-associated markers were used to study the ethephon-mediated effects on leaf senescence in detached sweet potato leaves. The chlorophyll contents and Fv/Fm values were drastically reduced, however, H(2)O(2) contents detected with diaminobenzidine (DAB) staining and a papain-like cysteine protease SPCP1 expression were significantly enhanced in ethephon-treated leaves compared to untreated dark control. In the presence of reduced glutathione, EGTA or cycloheximide, the reduction of chlorophyll contents and Fv/Fm values were alleviated, however, the induction or enhancement of H(2)O(2) contents and cysteine protease SPCP1 expression were repressed. Both calcium ionophore A23187 and glutathione synthase inhibitor, L-buthionine sulfoximide (BSO), remarkably induced SPCP1 expression in detached leaves, and the induction was also repressed by EGTA and reduced glutathione, respectively. The time effective for cycloheximide repression of SPCP1 expression was ca. 6 to 12 hours after ethephon treatment. In conclusion, ethephon-mediated effects on leaf senescence and gene expression in detached sweet potato leaves are significantly repressed by reduced glutathione, EGTA, and cycloheximide, respectively. These data suggest a possible involvement of oxidative stress, external calcium influx, and de novo synthesized proteins in association with ethephon signaling leading to leaf senescence and gene expression in sweet potato detached leaves.
Appears in Collections:	[Department of Biology ] journal articles

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