Evaluation of HPLC and MEKC methods for the analysis of Lipopeptide Antibiotic Iturin a Produced by Bacillus amyloliquefaciens

文化大學機構典藏 CCUR > 環境規劃暨生物資源學院 > 農學院 > 園藝暨生物技術學系 > 期刊論文 > Item 987654321/18844

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/18844

题名:	Evaluation of HPLC and MEKC methods for the analysis of Lipopeptide Antibiotic Iturin a Produced by Bacillus amyloliquefaciens
作者:	吳文希 Yerra Koteswara Rao Hung-Yuh Lin Yew-Min Tzeng
贡献者:	園藝系
关键词:	Bacillus amyloliquefaciens B128 High-performance liquid chromatography Micellarelectrokinetic chromatography Lipopeptide antibiotic Iturin A
日期:	2008-11
上传时间:	2011-01-19 11:12:04 (UTC+8)
摘要:	Two methods, high-performance liquid chromatography (HPLC) and micellar electrokinetic chromatography (MEKC), for analysis of multicomponent lipopeptide antibiotic iturin A are described. After conducting a series of optimizations, the HPLC separation of iturin A2, A3, A4, A5, A6, A7, and A8 isomers were achieved by a NPS RP-C18 column with an isocratic elution of acetonitrile/10 mM ammonium acetate (40:60, v/v), and flow rate of 1 mL/min. On the other hand, the MEKC separation achieved at 25℃ using an applied voltage of 20 kV, and background electrolyte consisting of 20 mM boric acid buffer (pH 8.72) with 50 mM sodium dodecyl sulfate (SDS) and 10% (v/v) acetonitrile. The calibration curve was linear to 20-200 μg/mL and50-500 μg/mL for HPLC and MEKC with R^2=0.9954 and R^2=0.9967, respectively. The detection limits of iturin A from the strain B128 cultivation medium were in the 2.5-7.0 μg/mL and 5.0-13.0 μg/mL ranges, and the reproducibility of within-day assay was 98.1 and 98.7%, for HPLC and MEKC, respectively (a UV detector was applied in the both cases). Both methods were selective, robust, and specific, allowing reliable quantification of iturin A, and could beuseful for clinical and biomedical investigations of related antibiotics.
關聯:	International Journal of Applied Science and Engineering v.6 n.2 P.85-96
显示于类别:	[園藝暨生物技術學系] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	446	检视/开启

在CCUR中所有的数据项都受到原著作权保护.

数据加载中.....