| 摘要: | 本研究以結球白菜’亞蔬1號’(‘ASVEG 1’)、’瑞農720’(‘RN720’)為試驗材料，在45天苗期給予0、6、24、48及72小時20 ℃未淹水、20 ℃淹水、40 ℃未淹水以及40 ℃淹水處理，測定葉片氣孔導度及葉綠素螢光，並萃取葉片蛋白質，以全自動二維蛋白質快速層析分離系統ProteomeLabTM PF 2D分析四種處理24與48小時之蛋白質體。結球白菜在淹水情況下，氣孔導度明顯下降；在淹水逆境下葉綠素螢光Fv/Fm值下降幅度不大，但在高溫逆境下Fv/Fm值降低，表示PSII效率下降，顯示結球白菜’亞蔬1號’及’瑞農720’對高溫逆境比對淹水逆境更加敏感。氣孔導度可作為結球白菜淹水逆境之生理指標，而葉綠素螢光可作為高溫逆境之生理指標。結球白菜’亞蔬1號’在高溫淹水24小時的情況下，發現UDP-glucose 6-dehydrogenase、PIK-kinase、OEE1與ribulose 1,5-bisphosphate carboxylase-oxygenase相似之蛋白質皆明顯的增加；3-hydroxy-3-methylglutaryl-1 CoA reductase、EIIAB、Nitrate reductase、 N-carbamoyl-L-amino acid amidohydrolase與CoB—CoM heterodisulfide reductase 1 subunit B，相似的蛋白質在處理後減少。20 ℃淹水48小時，ribonucleotide-diphosphate reductase alpha subunit、S-adenosylmethionine synthetase與RNA-dependent RNA polymerase相似的蛋白質表現增加；40 ℃未淹水48小時，rubisco ssu precursor與Ribulose bisphosphate carboxylase small chain相似的蛋白質表現增加；40 ℃淹水48小時，rubisco ssu precursor、Transcription elongation factor、Ribulose bisphosphate carboxylase small chain及Maf-like protein相似的蛋白質表現增加。結球白菜’瑞農720’在20℃淹水24小時下，Truncated hemoglobin-like protein與Ribulose bisphosphate carboxylase small chain的相似蛋白質增加；2-amino-3-ketobutyrate coenzyme A ligase與phosphoserine aminotransferase相似的蛋白質在處理後減少；在40℃未淹水24小時，Ribulose bisphosphate carboxylase small chain、NADH dehydrogease subunit 1、Ribulose bisphosphate carboxylase large chain precursor、F-box/Kelch-repeat protein及16 kDa calcium-binding protein相似的蛋白質表現增加；2-hydroxy-3-oxopropionate reductase、Tryptophan synthase alpha chain及Beta-microseminoprotein precursor相似的蛋白質在處理後減少。
The 45-days seedlings of Chinese cabbage ‘ASVEG 1’and ‘RN720’ were treated with 20℃/flooding, 20℃/ non-flooding, 40℃/flooding and 40℃/non-flooding for 0, 6, 24, 48 and 72 hours. Leaf stomatal conductance and chlorophyll fluorescence were determined. Leaf protein extracts were separated by ProteomeLabTM PF 2D. In the flooding treatments, stomatal conductance dropped. Fv/Fm also decreased in the 40℃ treatments. Chinese cabbage ‘ASVEG 1’and ‘RN720’ is more sensitive to heat stress than flooding stress. Stomatal conductance and chlorophyll fluorescence can be used as the physiological indicator of heat and flooding stress, separately. Chinese cabbage ‘ASVEG 1’ at 40 ℃/flooding for 24 hours, obvious increase occurred in proteins identical to UDP-glucose 6-dehydrogenase, PIK-kinase, OEE1 and ribulose 1,5-bisphosphate carboxylase-oxygenase, while decreased in proteins identical to 3-hydroxy-3- methylglutaryl-1 CoA reductase, EIIAB, nitrate reductase, N-carbamoyl- L-amino acid amidohydrolase and CoB-CoM heterodisulfide reductase 1 subunit B. Twenty ℃/flooding for 48 hours, obvious increase occurred in proteins identical to ribonucleotide- diphosphate reductase alpha subunit, S-adenosylmethionine synthetase and RNA- dependent RNA polymerase. Twenty ℃/non-flooding for 48 hr, obvious increases were the proteins identical to rubisco ssu precursor and Ribulose bisphosphate carboxylase small chain. Forty℃/non-flooding for 48 hours obvious increases were the proteins identical to rubisco ssu precursor, transcription elongation factor, ribulose bisphosphate carboxylase small chain and Maf-like protein. Chinese cabbage ‘RN720’ at 20 ℃/flooding for 24 hours, obvious increase occurred in proteins identical to Truncated hemoglobin-like protein and Ribulose bisphosphate carboxylase small chain, while decreased in proteins identical to 2-amino-3-ketobutyrate coenzyme A ligase and phosphoserine aminotransferase. Forty℃/non-flooding for 24 hours obvious increases were the proteins identical to Ribulose bisphosphate carboxylase small chain、NADH dehydrogease subunit 1、Ribulose bisphosphate carboxylase large chain precursor、F-box/Kelch-repeat protein and 16 kDa calcium-binding protein, while decreased in proteins identical to 2-hydroxy-3-oxopropionate reductase、Tryptophan synthase alpha chain and Beta-microseminoprotein precursor. |
