本研究主要係以丙烯酸(簡稱AA)為單體，過氧化二苯甲醯(簡稱 BPO)為化學起始劑，與聚丙烯不織布(簡稱PP不織布)進行接枝反應，並以聚麩胺酸(簡稱γ-PGA)交聯劑Glycerol polyglycidyl ether(簡稱GPE)、Di-glycerol polyglycidyl ether(簡稱DGPE)及Polyglycerol polyglycidyl ether(簡稱PGPE)將聚麩胺酸固定於不織布表面，接著再以戊二醛將幾丁聚醣 (Chitosan)固定於聚丙烯不織布。由結果顯示，隨著GPE、DGPE、PGPE交聯劑的用量、AA接枝率及固定溫度與固定時間的增加，經改質聚丙烯不織布上之聚麩胺酸固定率有上升的趨勢；另外，經聚麩胺酸固定之聚丙烯不織布再經幾丁聚醣固定後，其固定率會隨著戊二醛的用量及固定時間的增加而上升，但當達飽和狀態時，其固定率則不會再大幅度提升。至於抗菌方面，經γ-PGA及幾丁聚醣固定之改質聚丙烯不織布其抗菌率會隨著γ-PGA溶液濃度的增加而下降，但其下降幅度並不大，在聚麩胺酸溶液濃度為3%時，其抗菌率仍可達78.68%。另外，於抑菌圈測試中，樣本於聚麩胺酸溶液濃度為0.5%，幾丁聚醣溶液濃度為5%，固定時間為二十四小時，其抑菌圈可達12mm。由敷材之吸水膨潤性測試可看出敷材之吸水膨潤性會因為幾丁聚醣之特性而隨著緩衝溶液的pH值上升而下降；另外敷材之吸水膨潤性會隨著γ-PGA固定量的上升而上升，但固定γ-PGA及幾丁聚醣後，其敷材之吸水性則會隨著γ-PGA固定濃度的上升而下降；其擴散係數則呈現相同的情形。最後由大鼠傷口癒合測試及病理顯微觀察可知，經聚麩胺酸及幾丁聚醣混合固定之接枝聚丙烯不織布其癒合效果均較單一聚麩胺酸或幾丁聚醣之癒合效果為佳。
In this research, the polypropylene (abbr. PP) nonwoven fabric grafted with acrylic acid (abbr. AA) using benzoyl peroxide as a chemical initiator during the course of the polymerization has been investigated. Then the surface of PP nonwoven fabric was immobilized with a mixture of γ-Poly-glutamic acid(abbr. γ-PGA) and chitosan by glutaraldehyde and Glycerol polyglycidyl ether(abbr. GPE), Di-glycerol polyglycidyl ether(abbr. DGPE), Polyglycerol polyglycidyl ether(abbr. PGPE) as cross-linker.
The experimental results showed that the immobilization ratio increased with the increase of graft yield of AA monomer. Moreover, the immobilization ratio were found to increase with the increase of immobilization time and the add-on of glutaraldehyde、GPE、DGPE and PDPE aqueous solution. On the other hand, the antibacterial capacity decreases with the increase of the ratio of γ-PGA. When the ratio of γ-PGA is 3%, the antibacterial capacity is 78.68%. The inhibition zone of the sample (the ratio of γ-PGA is 0.5%，the ratio of chitosan is 5% and immobilization time is 24 hours) is 12mm. On the other hand, the swelling ratio decreases with the increase of the ratio of chitosan in the composites and the pH values in buffer solutions. However, the swelling ratio decreases with the increase of the ratio of γ-PGA in all pH of buffer solutions.
On skin wound healing of guinea-pig, it has been established that the immobilized with a mixture of γ-PGA and chitosan is better than only γ-PGA or chitosan. Another, the wound was covered by PP nonwoven with different monomer and immobilized with γ-PGA and chitosan completely healed for 21days, and fall off automatically. Therefore, PP nonwoven immobilized with γ-PGA and chitosan is a safe biomaterial for use as a wound dressing for skin healing.
