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請使用永久網址來引用或連結此文件:
https://irlib.pccu.edu.tw/handle/987654321/53190
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| 題名: | Isolation and Cultivation of Penicillium citrinum for Biological Control of Spodoptera litura and Plutella xylostella |
| 作者: | Nguyen, Hoang Chinh
Lin, Kuan-Hung
Nguyen, Thanh Phong
Le, Hong Son
Ngo, Kim Ngan
Pham, Dinh Chuong
Tran, Tuyet Nhung
Su, Chia-Hung
Barrow, Colin J.
林冠宏 |
| 貢獻者: | Department of Horticulture and Biotechnology, |
| 關鍵詞: | bio-pesticide
biocontrol;biorefinery
entomopathogenic fungi
Penicillium citrinum |
| 日期: | 2023-05 |
| 上傳時間: | 2024-03-12 15:28:50 (UTC+8) |
| 出版者: | Multidisciplinary Digital Publishing Institute |
| 摘要: | Biological control agents are a promising substitute for chemical pesticides in agricultural pest management. In this study, Penicillium sp. with high pathogenicity to the agricultural pests oriental leafworm moth (Spodoptera litura) and diamondback moth (Plutella xylostella) were isolated from naturally infected insects and grown on different agricultural residues as an inexpensive substrate for their sporulation. Ten strains of Penicillium (P.01~P.10) were identified as P. citrinum based on morphological features and molecular studies, with sequence analysis using an internal transcribed spacer region. Different fungal isolates exhibited a varying degree of pathogenicity against S. litura and Pl. xylostella, and strains P.04 and P.09 showed the highest pathogenicity to S. litura, with a mortality rate of 92.13% after 7 days of treatments, while strain P.06 resulted in the highest mortality of Pl. xylostella (100%) after 6 days of treatment. Moreover, among ten isolates infected with both S. litura and P. xylostella, P.06 showed potential virulence against S. litura and Pl. xylostella, with lethal time for 50% mortality (LT50) values of 4.5 days and 3.0 days, respectively. The ten isolates showed higher virulence to Pl. xylostella than to S. litura. The agro-industrial-based medium showed efficiency for the cultivation of isolates for sporulation on an industrial scale, suggesting that the newly isolated P. citrinum is a potential biological control agent for controlling insect pests and could be further developed for microbial pesticide production. |
| 關聯: | Fermentation |
| 顯示於類別: | [園藝暨生物技術學系] 期刊論文
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