高血脂症 (Hyperlipidemia)對於現代社會是一種常見的慢性病。人體內大部分總膽固醇 (total cholesterol, TCHO)的來源是從日常生活飲食中所獲取，隨著體內脂肪的過度累積，罹患高血脂症的人口比例也隨之上升，且已有研究表明高血脂症會緩慢誘導心血管疾病、動脈粥狀硬化等疾病。
在本次研究中，我們首先使用共沉澱法 (co-precipitation method)製備四氧化三鐵磁性奈米粒子，並使用海藻酸鈉 (sodium alginate)對磁性奈米粒子表面進行修飾，被修飾後的磁性奈米粒子 (alg-NH2-Fe3O4)之間的間隙將臨床用於治療高血脂症病患的藥物--非諾貝特 (fenofibrate, FEN)以及吉非羅齊 (gemfibrozil, GEM)進行捕捉，形成Nano FEN和Nano GEM，達到藥物奈米化的目的。接著利用動態光散射(dynamic light scattering, DLS)以及高效液相層析(high performance liquid chromatography, HPLC)進行物性及化性的分析。
此外，我們證明Nano FEN和Nano GEM可提升對高血脂症抑制的效果，我們將利用高脂飲食建立高血脂症的斑馬魚 (Danio rerio)動物模型進行生物體內各項指數測試，收集其三酸甘油脂 (TG)、總膽固醇 (TCHO)、血糖 (GLU)及高密度脂蛋白 (HDL-C)的血液分析數據，並搭配肝臟組織切片做為奈米化藥物可有效改善高血脂症的依據。
Hyperlipidemia is one of the common chronic diseases. The levels of triglycerides (TG) and total cholesterol (TCHO) in the blood are closely related to the level of hyperlipidemia. Generally, the cholesterol is from daily diet. There are increased population suffering from hyperlipidemia in modern society. Researches have showed that hyperlipidemia may slowly induce cardiovascular disease, atherosclerosis, which draw people’s attention in healthcare viewpoint.
In this study, we aimed to nanolized two commercial drugs for hyperlipidemia, fenofibrate (FEN) and gemfibrozil (GEM) to enhance the efficiency and reduce the side effects during treatment. We applied the co-precipitation method to prepare ferro ferric oxide (Fe3O4) nanoparticles, and then used sodium alginate to modify the surface of the magnetic nanoparticles. While there are some gaps formed between the magnetic nanoparticles, the drugs can be trapped in to the gap to form into Nano FEN and Nano GEM. The chemical and physical properties of Nano FEN and Nano GEM can be characterized by dynamic light scattering (dynamic light scattering, DLS) and high performance liquid chromatography (high performance liquid chromatography, HPLC).
In order to demonstrate Nano FEN and Nano GEM the efficiency on hyperlipidemia, we established a zebrafish (Danio rerio) model that has showed hyperlipidemia by specific high-fat diet. With the biochemical and histochemical analysis, we tried to prove that nanolized drugs showed significantly decrease the level of hyperlipidemia and may be lower the dosage of drug administration to ameliorate the adverse side effects.
