As the increasing of food delivery application service in Thailand, this research places focus to investigate the factors that impact on purchasing intention of working from home customers in food delivery application service at Bangkok. The research studies the relationship between working from home customer’s purchasing intention in food delivery application service and various factors including customers characteristics, marketing mix (4Ps), food delivery hygiene, social isolation and subject norm. The targeted respondent is Thai people who live in Bangkok who have experienced in WFH and using FDAS during COVID-19 period. The research instrument is an online questionnaire (Google Form) which developed in order to collect the data from 400 of targeted respondents. The obtained data analyzed by quantitative research method with statistic software SPSS statistic program. The result reveals that marketing mix (4Ps) in term of product, place and promotion positively impact on purchasing intention in food delivery application service. Moreover, foo delivery hygiene and social isolation also have positive influence. On the other hand, customers characteristics (gender, age, educational level, occupation, income), price and subject norm have no influence. This finding may help food application providers in develop marketing strategies for product, place, promotion, hygienic in food delivery and social isolation in order to serve the customer’s demand.