In this study, we used supercritical carbon dioxide fluid equipment for the extraction of essential oil from Coleus Amboinicus to investigate the effects of temperature and pressure on the yield of essential oil. This experiment uses a 32-experimental design, with pressure and temperature as the experimental condition factors, and each group of condition factors had three levels. The pressure conditions set in the experiment were 2100 psi, 2800 psi, and 3500 psi, and the temperature conditions were 40 °C, 50 °C, and 60 °C. Under a fixed flow rate of 150 NL/hr, each group was repeated three times, with a total of 27 groups.
At the end of the experiment, the data were analyzed by variance analysis (ANOVA) and second-order regression analysis, and the coefficients of the regression analysis were transferred to the MATLAB graphing software to draw reaction surfaces, and then Excel was used to draw the regression analysis of the estimated maximum yield. In the end, observing the significance of the interaction between the various factors helps get the best extraction conditions.The experimental results show that under the operating conditions pressure of 2800 psi and temperature of 40 ° C, the optimum yield of 2.49% can be obtained, and it is concluded that the temperature is the largest variable factor of the yield.
