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    题名: The origin and past demography of murine astrovirus 1 in laboratory mice
    作者: Su, CM (Su, Chia-Ming)
    Cheng, YC (Cheng, Ying-Chien)
    Wang, HY (Wang, Hurng-Yi)
    Hsieh, CH (Hsieh, Chia-Hung)
    Wan, CH (Wan, Cho-Hua)
    贡献者: 森保系
    关键词: Murine astrovirus
    MuAstV
    molecular characterization
    MuAstV strains in Taiwan
    intra-genotypic recombination
    MuAstV mutation rate
    日期: 2021
    上传时间: 2021-04-24 14:07:16 (UTC+8)
    摘要: Astroviruses are non-enveloped, positive-sense, ssRNA viruses and often associated with gastrointestinal diseases. Murine astrovirus (MuAstV) was first confirmed in a laboratory mouse colony in 2011. Although infected mice do not present significant clinical symptoms, the virus might interfere with research results. A recent surveillance has shown that MuAstV is highly prevalent in laboratory mice. The aims of the present study were to identify and characterize MuAstV strains as well as to investigate the prevalence rate of viral RNA in laboratory mice in Taiwan, and to estimate the origin and past population demography of MuAstVs. Based on molecular surveillance, MuAstV RNA was detected in 45.7% of laboratory mice (48/105) from seven of nine colonies. Three fully sequenced MuAstV strains, MuAstV TW1, TW2 and TW3, exhibited 89.1-94.4% and 89.1-90.0% nucleotide identities with the reference strains MuAstV STL1 and STL2, respectively. Phylogenetic analyses of the partial regions of the RNA-dependent RNA polymerase (RdRp) and capsid protein (CP) genes of 18 Taiwan strains along with other astroviruses revealed that there are three distinct lineages of mouse astrovirus, MuAstV1, MuAstV2 and mouse astrovirus JF755422. The mutation rates of MuAstV1 were 2.6x10(-4) and 6.2x10(-4) substitutions/site/year for the RdRp and CP regions, respectively. Based on the above molecular clock, the colonization of MuAstV1 in laboratory mice was between 1897 and 1912, in good agreement with the establishment of 'modern' laboratory mouse facilities. Since its initial infection, the population size of MuAstV1 has increased 15-60-fold, probably consistent with the increased use of laboratory mice. In conclusion, MuAstV1 has been associated with modern laboratory mice since the beginning, and its influence on research results may require further investigation.
    關聯: JOURNAL OF GENERAL VIROLOGY 卷冊: 102 期: 2 文獻號碼: 001520
    显示于类别:[森林暨自然保育學系 ] 期刊論文

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