Optimization of aqueous enzyme-assisted extraction of rosmarinic acid from rosemary (Rosmarinus officinalis L.) leaves and the antioxidant activity of the extract

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Title:	Optimization of aqueous enzyme-assisted extraction of rosmarinic acid from rosemary (Rosmarinus officinalis L.) leaves and the antioxidant activity of the extract
Authors:	Nguyen, HC (Nguyen, Hoang Chinh) Nguyen, HNT (Nguyen, Huynh Ngoc Truc) Huang, MY (Huang, Meng-Yuan) Lin, KH (Lin, Kuan-Hung) Pham, DC (Pham, Dinh-Chuong) Tran, YB (Tran, Yen Binh) Su, CH (Su, Chia-Hung)
Contributors:	園生系
Date:	2021-03
Issue Date:	2021-04-19 11:05:51 (UTC+8)
Abstract:	Rosemary (Rosmarinus officinalis L.) is a potential source of rosmarinic acid (RA). This study aimed to develop a novel aqueous enzymatic approach for extracting RA from rosemary leaves and evaluate the antioxidant activity of the RA-rich extract. The ability of several enzymes to release RA from plant tissues was investigated. Among the examined enzymes, cellulase A exhibited the highest extraction efficiency. Subsequently, aqueous cellulase A-assisted extraction was optimized. The optimal extraction conditions were as follows: an extraction time of 4.63 hr, a water-to-sample ratio of 28.69 ml/g, an enzyme concentration of 2.56%, and a temperature of 36.6 degrees C, with the maximum RA content of 13.97 mg/g. The RA-rich extract exhibited potent antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl radical with an IC50 value of 532.01 mu g/ml. Aqueous cellulase-assisted extraction is a green approach for RA extraction from rosemary leaves. Furthermore, the rosemary leaf extract is a promising antioxidant agent for future use. Practical applications RA is a potent antioxidant and is widely applied in the food and pharmaceutical industries. This study developed a new method involving the use of enzyme for ecofriendly extracting RA from rosemary leaves. The results indicated that the RA-rich extract is a potential antioxidant agent for future nutraceutical and pharmaceutical applications and the aqueous cellulase-assisted extraction is a practical and green process for RA extraction from rosemary leaves.
Relation:	JOURNAL OF FOOD PROCESSING AND PRESERVATION 卷冊: 45 期: 3 文獻號碼: e15221
Appears in Collections:	[Department of Horticulture] journal articles

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