| 摘要: | 佛甲草屬為一或多年生草本植物，屬於景天科(Crassulaceae)佛甲草屬(Sedum)的植物，收錄於本草綱目草部，第二十卷草之九(石草類一十九種)，在中草藥中被認為具有清熱解毒、利濕、止血的功效。在台灣原住民藥用植物及中國大陸地區，均有不同的藥用功效，可見得佛甲草治療功效廣泛並且用藥安全，具備研究開發價值。本研究目的是分析台灣原生及特有的佛甲草屬植物為台灣佛甲草(S. formosanum)與玉山佛甲草(S. morrisonense)在高溫逆境對其生理、生化的反應之影響。試驗材料在採樣後盆植，將30天齡植株置於生長箱，分別以日溫/夜溫10/5℃、15/10℃、25/25℃ (控制組)、30/15℃、35/20℃、40/25℃處理0 (控制組)、3、12、24、36、48與72 h，每日光照12小時，比較並探討在不同逆境處理下，其抗氧化性(DPPH自由基清除率、螯合亞鐵能力與總還原力)、總酚含量及抗氧化酵素SOD活性之變化。並利用ICP-MS分析其植株是否有重金屬殘留或其他元素之超累積特性，以評估其成為中草藥或特殊地景設計民俗植物與回復地力，提高台灣原生佛甲草族群復育的可能性。結果發現24h玉山佛甲草在30/15℃之DPPH、氧化價還原力及螯合亞鐵均顯著高於台灣佛甲草，且在15/10℃總酚含量也高於台灣佛甲草。此外，玉山佛甲草與台灣佛甲草在溫度逆境下，其SOD活性並沒有隨之改變。在ICP-MS重金屬檢測中，所有供試樣本皆未發現有重金屬殘留，故評估玉山佛甲草可作為中草藥之可能與成為可食地景設計植物。
Sedum is a type of annual or perennial herbs, from the Crassulaceae family, Sedum genus. It is recorded in the Compendium of Materia Medica herb section, Volume 20 Herb entry No.9; and are generally considered to have detoxification, diuresis, and hemostasis effects in Chinese Herbology. Its medicinal recipes are varied both as a type of Taiwan aboriginal herb and are spread throughout the Chinese mainland. Sedum have far-reaching medical potential and as safe medication, truly deserve research and marketing attention. This research focus on Taiwan’s indigenous Sedum population: S. Formosanum and S. morrisonense; more specifically, their biological and biochemical reaction to high temperature stress. The test samples are pot planted after sampling, the 30 days old plants are then situated inside a growth chamber, processed with varying day/night temperature differences, namely: 10/5℃, 15/10℃, 25/25℃ (Control Group), 30/15℃, 35/20℃, 40/25℃; and time differences: 0 (Control Group), 3, 12, 24, 36, 48, and 72hours. All samples are subjected to 12 hours of sunlight daily. This research explore how different stress variables, could result in different antioxidant levels (DPPH free radicals clearance rate, Fe2+ chelation ability, and total reducing power), total phenol content, and anti-oxidant enzyme SOD activity fluctuations for the plant. ICP-MS is also utilized to analyze whether the test subject could have heavy metal residues and hyperaccumulation of other elements. This evaluation is setup to see if the plant could have potential as a Chinese herbal medicine or special landscape design ethnobotanical element, to explore its soil fertility properties, and to raise restoration possibilities of Taiwan’s indigenous Sedum population.The conclusion of the test results: 24H Sedum Morrionense under 30/15℃’s DPPH level have significant higher oxidation reduction power and Fe2+ chelation levels than Sedum formosanum; significantly, the total phenol content were also higher than Sedum formosanum under 15/10℃. In addition, SOD activities did not change for either native species under high temperature stress tests. The ICP-MS test scores yielded no heavy metal residues in all lab samples. Hence, this evaluation value Sedum Morrionense both as potential Chinese Herbal Medicine and as well as an edible landscape design element. |
