Establishment of an Immunocompetent Metastasis Rat Model with Hepatocyte Cancer Stem Cells

CCUR > College of Natural Science and Engineering > College of Science > Department of Biology > journal articles > Item 987654321/49217

Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/49217

Title:	Establishment of an Immunocompetent Metastasis Rat Model with Hepatocyte Cancer Stem Cells
Authors:	Wu, S (Wu, Semon) Tseng, IC (Tseng, I-Chieh) Huang, WC (Huang, Wen-Cheng) Su, CW (Su, Cheng-Wen) Lai, YH (Lai, Yu-Heng) Lin, C (Lin, Che) Lee, AYL (Lee, Alan Yueh-Luen) Kuo, CY (Kuo, Chan-Yen) Su, LY (Su, Li-Yu) Lee, MC (Lee, Ming-Cheng) Hsu, TC (Hsu, Te-Cheng) Yu, CH (Yu, Chun-Hsien)
Contributors:	生科系
Keywords:	hepatocyte cancer stem cell cell renew drug resistance metastasis xenografts
Date:	2020-12
Issue Date:	2021-01-19 09:53:31 (UTC+8)
Abstract:	Simple Summary Cancer stem cells (CSCs) are considered responsible for the maintenance, metastasis, and recurrence of various tumors. Here, we isolated a novel CSCs, TW-1, which can self-renew, differentiate and resistant to sorafenib. We also established novel orthotopic and metastasis models in immunocompetent rats with TW-1. These immunocompetent animal model can provide a normal physiological condition for anti-cancer drug screening. Using machine learning, we predicted HCC (AUCs > 0.9) with eight biomarkers including 6 highly expressed in TW-1/HTC and 2 well-known biomarkers from recent HCC studies. In conclusion, our results showed that TW-1 was a novel rat CSC line, and the animal model established with TW-1, is not only useful for the study of metastasis of HCC but also preclinical cancer drug screening. Hepatocellular carcinoma (HCC) is one of the leading causes of cancer mortality. Cancer stem cells (CSCs) are responsible for the maintenance, metastasis, and relapse of various tumors. The effects of CSCs on the tumorigenesis of HCC are still not fully understood, however. We have recently established two new rat HCC cell lines HTC and TW-1, which we isolated from diethylnitrosamine-induced rat liver cancer. Results showed that TW-1 expressed the genetic markers of CSCs, including CD133, GSTP1, CD44, CD90, and EpCAM. Moreover, TW-1 showed higher tolerance to sorafenib than HTC did. In addition, tumorigenesis and metastasis were observed in nude mice and wild-type rats with TW-1 xenografts. Finally, we combined highly expressed genes in TW-1/HTC with well-known biomarkers from recent HCC studies to predict HCC-related biomarkers and able to identify HCC with AUCs > 0.9 after machine learning. These results indicated that TW-1 was a novel rat CSC line, and the mice or rat models we established with TW-1 has great potential on HCC studies in the future.
Relation:	CANCERS 卷冊: 12 期: 12 文獻號碼: 3721
Appears in Collections:	[Department of Biology ] journal articles

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	176	View/Open

View Licence

Loading...