Development of a Modified Three-Day T-maze Protocol for Evaluating Learning and Memory Capacity of Adult Zebrafish

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Title:	Development of a Modified Three-Day T-maze Protocol for Evaluating Learning and Memory Capacity of Adult Zebrafish
Authors:	Hieu, BTN (Bui Thi Ngoc Hieu) Anh, NTN (Nguyen Thi Ngoc Anh) Audira, G (Audira, Gilbert) Juniardi, S (Juniardi, Stevhen) Liman, RAD (Liman, Rhenz Alfred D.) Villaflores, OB (Villaflores, Oliver B.) Lai, YH (Lai, Yu-Heng) Chen, JR (Chen, Jung-Ren) Liang, ST (Liang, Sung-Tzu) Huang, JC (Huang, Jong-Chin) Hsiao, CD (Hsiao, Chung-Der)
Contributors:	化學系
Keywords:	passive avoidance ZnCl2 spatial memory T-maze leptin a zebrafish
Date:	2020-02
Issue Date:	2020-12-11 14:21:32 (UTC+8)
Abstract:	A T-maze test is an experimental approach that is used in congenital research. However, the food reward-based protocol for the T-maze test in fish has low efficiency and a long training period. The aim of this study is to facilitate the T-maze conditions by using a combination of the principles of passive avoidance and a spatial memory test. In our modified T-maze settings, electric shock punishment (1-2 V, 0.3-0.5 mA) is given at the left arm, with a green cue at the right arm. Also, the depth of both arms of the T-maze was increased. The parameters measured in our T-maze design were latency, freezing time, and time spent in different areas of the T-maze. We validated the utility of our modified T-maze protocol by showing the consistent finding of memory impairment in ZnCl2-treated fish, which has been previously detected with the passive avoidance test. In addition, we also tested the spatial memory performance of leptin a (lepa) mutants which displayed an obesity phenotype. The results showed that although the learning and memory performance for lepa KO fish were similar to control fish, they displayed a higher freezing behavior during the training phase. In conclusion, we have established a modified T-maze protocol that can be used to evaluate the anxiety, learning, and memory capacity of adult zebrafish within three days, for the first time.
Relation:	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 卷冊: 21 期: 4 文獻號碼: 1464
Appears in Collections:	[Department of Chemistry & Graduate Institute of Applied Chemistry ] journal articles

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