文化大學機構典藏 CCUR:Item 987654321/48871
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    题名: Regulation of micoRNA2111 and its target IbFBK in sweet potato on wounding
    作者: Weng, ST (Weng, Shiau-Ting)
    Kuo, YW (Kuo, Yun-Wei)
    King, YC (King, Yu-Chi)
    Lin, HH (Lin, Hsin-Hung)
    Tu, PY (Tu, Pin-Yang)
    Tung, KS (Tung, Kuei-Shu)
    Jeng, ST (Jeng, Shih-Tong)
    贡献者: 園生系
    关键词: Sweet potato
    Wounding
    Small RNA deep sequencing
    miR2111
    F-box/kelch repeat protein (FBK)
    SCF complex
    日期: 2020-03
    上传时间: 2020-12-10 15:10:09 (UTC+8)
    摘要: Plant microRNAs (miRNAs) are non-coding RNAs, which are composed of 20-24 nucleotides. MiRNAs play important roles in plant growth and responses to biotic and abiotic stresses. Wounding is one of the most serious stresses for plants; however, the regulation of miRNAs in plants upon wounding is not well studied. In this study, miR2111, a wound-repressed miRNA, identified previously in sweet potato (Ipomoea batatas cv Tainung 57) by small RNA deep sequencing was chosen for further analysis. Based on sweet potato transcriptome database, F-box/kelch repeat protein (IbFBK), a target gene of miR2111, was identified. IbFBK is a wound-inducible gene, and the miR2111-induced cleavage site in IbFBK mRNA is between the 10th and 11th nucleotides of miR2111. IbFBK is a component of the E3 ligase SCF (SKP1-Cullin-F-box) complex participating in protein ubiquitination and degradation. The results of yeast two-hybrid and bimolecular fluorescence complementation assays demonstrate that IbFBK was conjugated with IbSKP1 through the F-box domain in IbFBK N-terminus to form SCF complex, and interacted with IbCNR8 through the kelch-repeat domain in IbFBK C-terminus. The interaction of IbFBK and IbCNR8 may lead to the ubiquitination and degradation of IbCNR8. In conclusion, the suppression of miR2111 resulted in the increase of IbFBK, and may regulate protein degradation of IbCNR8 in sweet potato responding to wounding.
    關聯: PLANT SCIENCE 卷冊: 292 文獻號碼: 110391
    显示于类别:[園藝暨生物技術學系] 期刊論文

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