Expression Fluctuations of Genes Involved in Carbohydrate Metabolism Affected by Alterations of Ethylene Biosynthesis Associated with Ripening in Banana Fruit

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题名:	Expression Fluctuations of Genes Involved in Carbohydrate Metabolism Affected by Alterations of Ethylene Biosynthesis Associated with Ripening in Banana Fruit
作者:	Xia, Y (Xia, Yan) Chiu, CH (Chiu, Chien-Hsiang) Do, YY (Do, Yi-Yin) Huang, PL (Huang, Pung-Ling)
贡献者:	生科所
关键词:	carbohydrate metabolism transcriptome fruit ripening RNA interference 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase
日期:	2020-09
上传时间:	2020-11-05 09:56:58 (UTC+8)
摘要:	The banana is a typical climacteric fruit that undergoes ethylene dependent ripening. During fruit ripening, ethylene production triggers a developmental cascade that results in a series of physiological and biochemical changes. The fruit transcriptomes of untransformated wild-type (WT) and RNAi transgenic banana plants forMh-ACO1andMh-ACO2have been previously sequenced and analyzed, and most of the differentially expressed genes were enriched in 'carbon fixation in photosynthetic organism', 'cysteine and methionine metabolism', 'citrate cycle (tricarboxylic acid cycle, TCA cycle)', and 'starch and sucrose metabolism' based on Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation. In this research, we investigated the expression fluctuations of genes involved in carbohydrate metabolism affected by alterations of ethylene biosynthesis associated with ripening in banana fruits. Expression profiles of sucrose synthase, sucrose phosphate synthase, neutral invertase, and acidic invertase/beta-fructofuranosidase, as analyzed by Avadis and Trinity, showed that both analyses were complementary and consistent. The overall gene expression tendency was confirmed by the implementation of quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) with mRNAs of banana fruits inMh-ACO1andMh-ACO2RNAi transgenic plants. These results indicated that altered expression of genes associated with ethylene biosynthesis strongly influenced the expression levels of genes related to starch and sucrose metabolism, as well as the glycolysis pathway in ripening banana fruits.
關聯:	PLANTS-BASEL 卷冊: 9 期: 9 文獻號碼: 1120
显示于类别:	[生物科技研究所 ] 期刊論文

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