In cell culture, medium and serum provide essential nutrients and growth factors for cell growth. Since the nutrient content is differ in diverse culture medium, it is important to predetermine the appropriate media for experimental design and cell culture. In this study, we examined the effects of different culture medium and serum on the growth performance of non-tumor mouse liver cells (FL83B). Four different culture medium, DMEM + 10% FBS, DMEM + 10% NCS, DMEM-F12 + 10% FBS and DMEM-F12 + 10% NCS, were evaluated for their effects on cell growth, which was estimated by surface covering rate at different times. The changes in cell-cover area in flasks were determined by image J and analyzed by SAS. The results suggested that DMEM-F12-FBS had the greatest efficiency on cell proliferation, prolong cell growth and survival, but cell-cover area decreased after day 7 when cells cultured in DMEM-F12, whereas cover areas of cells cultured in DMEM-FBS and DMEM-NCS continued to increase. For nutritional and hormonal treatments, we found that DMEM + 10%FBS might be most suitable for providing steady growth and to allow sufficient time to response to treatments.
