| 摘要: | 本論文主要是要製備一種具有產生氧氣功能的過氧化鈣/海藻膠/金奈米棒奈米複合材料，並可以將其應用於光熱治療與光動力治療上，首先，先製備出金奈米棒做為藥物載體，利用紫外光-可見光光譜儀測得金奈米棒特徵波長為520 nm和800 nm，同時利用表面電位測定儀來量測材料的表面電荷變化，來證實海藻膠有確實修飾在金奈米棒表面；接著將光敏劑包覆於海藻膠/金奈米棒，再加入氯化鈣使鈣離子與海藻膠螯合；最後加入雙氧水將鈣離子轉換成遇水時能產生氧氣的過氧化鈣。此奈米複合材料表面的過氧化鈣可與水反應，進而產生氧氣，在腫瘤區域釋放出氧氣，使腫瘤區域的含氧濃度提高，改善腫瘤區域的缺氧微環境，並同時搭配包覆光敏劑的海藻膠/金奈米棒奈米材料與光動力治療發展出一種新式可殺死癌細胞的策略，也同時可達到更有效率的腫瘤治療效果，希望未來能將此材料運用於與腫瘤相關的醫療方面上。利用光動力治療來殺死癌細胞是一種非侵入式且有效的治療方式，同時為了解決腫瘤區域的缺氧而限制光動力治療的問題，我們製備出一種可產生氧氣，且同時攜帶光敏劑的金奈米棒複合材料來改善缺氧環境。
The aim of this study is to prepare an oxygen-generated nanocomposite (CaO2/ICG/alginate/Au nanorod) and then apply for photothermal (based on Au NRs) and photodynamic therapies (based on ICG). First, Au nanorods (NRs) with as aspect ratio of 3.8 were synthesized and then alginate was used to cover the surface of Au NRs with electrostatic interaction. Indocyanine green(ICG) was selected as a photosensitizer for photodynamic therapy in this study.Alginate-conjugated Au NRs could encapsulate ICG and then Ca2+ was used to cover the surface of alginate-conjugated Au NRs (alginate-Au NRs) to prevent ICG leaching from material surface. Final, Ca2+-alginate-Au NRs could be transfer to CaO2-alginate-Au NRs after Ca2+ reacts with H2O2. CaO2 can react with water to produce O2. When Ca2+-alginate-Au NRs exist in tumor region, O2 can be generated and then the hypoxia microenvironment will be improved (O2level increase), resulting in better therapeutic efficiency. CaO2/ICG/alginate/Au NRs combined photothermal therapy, photodynamic therapy and O2 generation to treat tumor. This is a new strategy for tumor treatment with high therapeutic efficiency, as compared to current tumor treatment methods. |
