| 摘要: | 蘇丹紅(Sudan)為人工合成之紅色工業染料，因其具有遺傳毒性，世界各國大多禁止Sudan作為食品添加劑使用，但由於其成本低廉且染色力強，因此常見非法使用於蛋雞、蛋鴨飼料以及辣椒和番茄製品中，嚴重危害消費者的健康。目前對於檢測出Sudan的蛋品生產者皆是以全面撲殺動物為處理方案，若了解Sudan在動物體內的代謝途徑與時間，可推測其安全性並避免全面撲殺以減少非必要的動物犧牲。本研究給予蛋雞高劑量（40mg/Kg）與低劑量（4mg/Kg）之Sudan，餵飼一週後恢復餵飼正常飼料，並採其蛋進行蛋品質分析，另採集血、肝臟、脂肪及蛋，觀察各階段Sudan與Sudan代謝物殘留量。結果顯示蛋重、蛋殼強度與豪氏單位並無顯著差異，高劑量組蛋黃色度在餵飼第二日達到15，並在停用Sudan20日後恢復正常，低劑量組蛋黃色度最高達到12，並在停用Sudan14日後恢復正常。蛋黃內Sudan之最高殘留量於高劑量與低劑量處理組分別為2.78和0.7 mg/kg。在停用20日之後，蛋黃、肝臟、血液與脂肪皆未能檢出Sudan IV 與其代謝物。研究發現蛋黃色度恢復速度與Sudan 代謝速度相似，因此若未來有蛋雞或蛋鴨場被檢出含Sudan之雞蛋或鴨蛋，或可從蛋黃色度進行Sudan 殘留的快速簡易追蹤，但安全殘留之檢測仍須以儀器檢測結果為準。
Sudan is a carcinogenic industrial azo-dye and is restricted for use in food. However, it has been detected in food on several occasions, such as in paprika and in egg yolk. This study was designed to simulate the transfer of Sudan to eggs and tissues after its administration to laying hens. Three groups of laying hens received feed containing Sudan IV at 0 mg/kg, 4mg/kg and 40 mg/kg. One week after feeding, normal feeding was resumed, and eggs were collected for egg quality analysis, blood, liver, fat and eggs were collected and analyzed for the concentrations of Sudan and it’s metabolites. The results showed that there was no significant difference in egg weight, eggshell strength, and Haugh unit. In the high-dose group, Roche color fan scale reached 15 on the second day of feeding and returned to normal at 20 days after withdrawn from feed. The highest level of Roche color fan scale in the low-dose group reached 12 and returned to normal at 14 days after withdrawn from feed. The highest Sudan concentration in the egg yolk was 2.78 and 0.7 mg/kg in the high-dose and low-dose treatment groups, respectively. At 20 days after withdrawn from feed, Sudan IV and its metabolites were not detected in egg yolk, liver, blood and fat. In conclusion, findings of ow study suggests that the resume of egg color seems to correlate with the clearance rate of sudan in tissues. Thus, observation of egg yolk color change might be a quicker and simple way to determine the clearance of Sudan after its contamination in eggs. However, residue concentration still need to be analyzed by certain high tech equipment to ensure the safeness of the food. |
