文化大學機構典藏 CCUR:Item 987654321/41969
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    Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/41969


    Title: Rapid screening of Mycobacterium tuberculosis complex (MTBC) in clinical samples by a modular portable biosensor
    Authors: Prabowo, BA (Prabowo, Briliant Adhi)
    Chang, YF (Chang, Ying-Feng)
    Lai, HC (Lai, Hsin-Chih)
    Alom, A (Alom, Azharul)
    Pal, P (Pal, Parthasarathi)
    Lee, YY (Lee, Yu-Ying)
    Chiu, NF (Chiu, Nan-Fu)
    Hatanaka, K (Hatanaka, Koji)
    Su, LC (Su, Li-Chen)
    Liu, KC (Liu, Kou-Chen)
    Contributors: 物理系
    Keywords: PLASMON RESONANCE IMMUNOSENSOR
    REAL-TIME PCR
    LABEL-FREE
    NUCLEIC-ACIDS
    IDENTIFICATION
    ASSAY
    DNA
    DIAGNOSIS
    MIESCHER,FRIEDRICH
    SENSITIVITY
    Date: 2018-01
    Issue Date: 2019-01-23 11:46:01 (UTC+8)
    Abstract: Mycobacterium tuberculosis complex (MTBC) is the well-known causative agents of tuberculosis (TB). Transmission of bacteria via airborne routes makes infection control difficult. Hence, rapid and accurate identification of TB from clinical sputum samples is essential for minimizing the spread of the disease. However, traditional bacterial identification methods for TB are time consuming and labor intensive. The aim of this study is to propose a straightforward and rapid screening platform for MTBC using a portable organic light-emitting diode- (OLED-) based surface plasmon resonance (SPR) biosensor integrated with a nested PCR technique. Experimentally, the limit of detection (LOD) for MTBC PCR products measurement was estimated to be around 63 pg/mL. In addition, the biosensor can successfully differentiate MTBC from other nontuberculous mycobacterium strains. In 600 clinical sputum specimens, the sensitivity and specificity were 96.6% (86/89) and 98.4% (503/511), respectively. These results indicate that the portable OLED-based SPR biosensor may be taken into account as a suitable and convenient tool employed in the remote area and developing country for diagnosing TB diseases. (C) 2017 Published by Elsevier B.V.
    Appears in Collections:[Department of Physics ] journal articles

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