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https://irlib.pccu.edu.tw/handle/987654321/41950
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| Title: | Study of Chlorophyll-related Compounds from Dietary Spinach in Human Blood |
| Authors: | Chao, PY (Chao, Pi-Yu)
Huang, MY (Huang, Meng-Yuan)
Huang, WD (Huang, Wen-Dar)
Lin, KH (Lin, Kuan-Hung)
Chen, SY (Chen, Shiau-Ying)
Yang, CM (Yang, Chi-Ming) |
| Contributors: | 保健營養學系 |
| Keywords: | ANTIOXIDANT ACTIVITY
DIGESTIVE STABILITY
PYROPHEOPHORBIDE-A
INTESTINAL-CELL
DERIVATIVES
CANCER
PHARMACOKINETICS
TUMOR
MICELLARIZATION
PHEOPHORBIDE |
| Date: | 2018 |
| Issue Date: | 2019-01-22 14:41:56 (UTC+8) |
| Abstract: | Human bioavailability data on chlorophyll (Chl) is very limited. The distribution of Chl-related compounds (CRCs) derived from dietary spinach was investigated in human blood. Eight healthy adults, aged from 21 to 61 year-old, consumed 1.2 kg of just-boiled fresh spinach after an 8-h overnight fast. Before and then 3 h after consuming the spinach, blood samples were taken from each participant. Freeze-dried blood samples were prepared, and 80% acetone was added for grinding. Eight peaks were found in the blood using high-performance liquid chromatography (HPLC), and the main CRCs in the samples were pheophytin (Phe) and pheophorbide (Pho) derivates. Compared to a fasted state, markedly higher levels of blood CRCs were detected in all subjects, except that Pho metabolites were not found in two subjects. No significant differences were seen in most of the peaks between males and females; however, relatively higher CRCs levels were observed in females, particular of Pho derivates. In addition, the blood contained significantly higher levels of Phe in the 36 similar to 61-year-old group than in the 21 similar to 35-year-old group. These results suggest that the conversion of Chls to CRCs is a rapid process, and Chls obtained by ingestion can be absorbed by the human body. |
| Appears in Collections: | [Department of Food and Nutrition ] journal articles
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